晚期肺癌患者化疗前后细胞免疫功能及T细胞分泌细胞因子的变化  

Changes of cellular immune function and cytokines secreted by T lymphocyte after chemotherapy in patients with advanced lung cancer

作　　者：曹喆[1,2] 何玲[2] 周剑[2] 章永[2] 梅齐[3] 

机构地区：[1]湖北科技学院临床医学肿瘤和核医学教研室,湖北科技学院附属第一医院肿瘤科,湖北咸宁437000 [2]咸宁市中心医院,湖北科技学院附属第一医院肿瘤中心,湖北咸宁437000 [3]华中科技大学同济医学院附属同济医院肿瘤科,武汉430030

出　　处：《感染．炎症．修复》2017年第2期90-93,共4页Infection Inflammation Repair

基　　金：湖北省卫生计生科研基金资助(WJ2015MB233)

摘　　要：目的:探讨晚期肺癌患者化疗前后外周血中T细胞免疫功能及其分泌细胞因子水平的变化。方法:通过流式细胞仪检测57例晚期肺癌患者和22例健康体检者CD4^+T淋巴细胞和CD8^+T淋巴细胞在外周血单核细胞(PBMC)中所占比例以及外周血CD4^+T细胞分泌细胞因子水平的变化。结果:晚期肺癌患者化疗前CD4^+T细胞在PBMC中所占比例、CD4^+T细胞与CD8^+T细胞比值均低于健康人(P<0.001),化疗后均较化疗前明显升高(P<0.01或P<0.001);化疗前CD8^+T细胞在PBMC中所占比例高于健康人(P<0.001),而于化疗后较化疗前明显下降(P<0.05)。治疗前后患者血清中CD4^+T细胞分泌Th1细胞因子(IFN-γ、TNF-α、IL-2)和Th2细胞因子(IL-4、IL-6、IL-10)水平均无明显变化(P>0.05)。结论:化疗能够增加肺癌患者外周血中CD4^+T细胞所占比例,但对Th1、Th2所分泌的相关细胞因子水平影响不大。Objective： To investigate the changes of cellular immune function and cytokines secreted by T lymphocyte after chemotherapy in patients with advanced lung cancer. Methods： Flow cytometry was performed to detect the proportions of CD4＋T and CD8＋T lymphocytes in peripheral blood mononuclear cells （PBMCs） as well as the level of cytokines secreted by CD4＋T cells. Results： The proportion of CD4＋T in PBMCs and the ratio of CD4＋T/CD8＋T were lower in patients with advanced lung cancer than those of the control group （P〈0.001） before chemotherapy, and increased after chemotherapy （P〈0.01 or P〈0.001）. The proportion of CD8＋T in PBMCs was higher in patients with advanced lung cancer than those of the health controls （P〈0.001） before chemotherapy, then it decreased after chemotherapy （P〈0.05）. However, no obvious changes were found in the levels of cytokines secreted by Th1 （such as IFN-y, TNF-a and IL-2） or Th2 lymphocyte （IL-4, IL-6 and IL-10） in patients. Conclusions： Chemotherapy may increase the proportion of CD4＋T lymphocytes in PBMCs in patients with advanced lung cancer, but shows little effect on the changes of cytokines secreted by Th1 or Th2.

关 键 词：肺癌 化疗 免疫细胞 细胞因子 

分 类 号：R446.63[医药卫生—诊断学]