新疆红枣软腐病病原菌的鉴定  

Identification of the Pathogen of Jujube Soft Rot in Xinjiang

作　　者：白剑宇[1,2] 宋峰惠 吴正保[2] 刘正兴[3] 崔燕华[3] 史彦江[2] 

机构地区：[1]新疆农业大学林学与园艺学院博士流动站,乌鲁木齐830052 [2]新疆林业科学院经济林研究所,乌鲁木齐830063 [3]阿克苏地区农业技术推广中心,新疆阿克苏843000

出　　处：《新疆农业科学》2017年第8期1475-1480,共6页Xinjiang Agricultural Sciences

基　　金：中国博士后科学基金面上项目“新疆红枣黑斑病菌的田间追踪检测技术研究”(2014M552565XD);新疆维吾尔自治区林业发展补助资金项目“南疆红枣主产区红枣主要病害普查鉴定及田间追踪监测技术研究”(XJLKY2016056)~~

摘　　要：【目的】鉴定枣软腐病的致病病原,为有针对性的开展病害防治奠定基础。【方法】采用病原菌常规分离技术获取疑似病原物,通过形态学特征和回接验证对病原进行鉴定;利用PCR技术获取病原菌的ITS基因的序列片段,通过与Gen Bank中已有的相关序列进行比对分析,构建遗传进化树,结合形态学鉴定和致病性实验验证,鉴定病原。【结果】新疆枣软腐病病原的形态学特征与米根霉(Rhizopus oryzae)相似。枣软腐病的病原与Gen Bank中已知的米根霉菌株的ITS序列显示99%~100%的相似性,在遗传进化树分析中并聚在同一组。【结论】基于形态学特征、致病性试验以及分子鉴定结果,新疆枣软腐病病原被鉴定为Rhizopus oryzae。[Objective] The pathogen of soft rot disease of jujube was accurately identified so as to lay a foundation for disease prevention and control.[Method] The suspected pathogens were obtained by the con-ventional isolation technology and were preliminarily identified by morphological features and loop validation. The fragments of ITS gene of the suspected pathogens were obtained by the PCR technique. When the related trains of Rhizopus oryzae in GenBank were compared and analyzed, the genetic evolutionary tree was construc-ted and the pathogen was identified by morphological identification and pathogenicity experiments.[Result] According to the morphological characteristics and the pathogenicity test results, the morphological characteris-tics of Rhizopus soft rot pathogen of Xinjiang jujube were similar to that of Rhizopus oryzae. The phylogenetic analysis of the pathogen showed that the ITS gene of pathogen of jujube soft rot showed 99 % to 100 % simi-larity with part ITS gene of Rhizopus oryzae in GenBank, and in the analysis of phylogenetic tree, they were clustered in the same group.[Conclusion] Based on the morphological characteristics, pathogenicity test and molecular identification of the pathogen, the pathogen of jujube soft rot was identified as Rhizopus oryzae.

关 键 词：枣软腐病 RDNA-ITS 病原鉴定 遗传进化树分析 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]