致痉挛性杀鼠剂气相色谱质谱联用快速确认法  

Quick confirmation of spasms-causing raticide by gas chromatography-mass spectrometry

作　　者：张英[1] 蔡志斌[1] 徐小燕[1] 赖建辉[1] 刘金明[1] 刘俩燕[1] 

机构地区：[1]深圳市龙岗区疾病预防控制中心检验科,广东深圳518172

出　　处：《职业与健康》2017年第14期1906-1909,共4页Occupation and Health

基　　金：深圳市卫生计生系统科研项目(201303222)

摘　　要：目的建立食品和生物体等中毒样本中毒鼠强、氟乙酰胺、氟乙酸钠3种致痉挛性杀鼠剂的气相色谱质谱联用仪(GC-MS)快速确认方法。方法样品经液-液萃取、氮吹浓缩后毒鼠强、氟乙酰胺直接上GC-MS进行定性确认与定量检测,氟乙酸钠需要进一步微波衍生后上GC-MS测试。结果该方法氟乙酰胺与毒鼠强的定量下限均为0.2 mg/kg,氟乙酸钠定量下限为1.0 mg/kg,3种杀鼠剂的相对标准偏差(RSD)在2.27%~8.54%之间,回收率在63.3%~92.62%之间,线性相关系数r均大于0.999。结论该方法简单、快速、准确可靠,适用于食品和生物体等中毒检材中毒鼠强、氟乙酰胺、氟乙酸钠3种致痉挛性杀鼠剂的快速气象色谱质谱确证。[Objective]To establish a method for rapid determination of 3 kinds of spasms-causing raticide（tetramine,fluoroacetamide and sodium fluroacetate）in poisoning samples such as food and biological fluids by gas chromatography-mass spectrometry（GC-MS）.[Methods]The samples were extracted by liquid-liquid extraction and concentrated by nitrogen. The qualitative confirmation and quantitative detection of tetramine and fluoroacetamide were performed by GC-MS directly. The sodium fluroacetate were determined by GC-MS after microwave assisted derivatization.[Results]The lower limit of quantitation for tetramine and fluoroacetamide were both 0.2 mg/kg,and that for sodium fluroacetate was 1.0 mg/kg. The relative standard deviation（RSD） of 3 kinds of raticide was 2.27%-8.54%,the recoveries were between 63.3% and 92.62%,and the correlation coefficients（r）for the linear equation were above 0.999.[Conclusion] The method is simple,rapid,accurate and sensitive,which is suitable for the quick confirmation of spasms-causing raticide（tetramine,fluoroacetamide and sodium fluroacetate） in poisoning samples such as food and biological fluids by GC-MS.

关 键 词：气相色谱质谱联用仪 致痉挛性杀鼠剂 氟乙酰胺 微波衍生 

分 类 号：R115[医药卫生—公共卫生与预防医学]