Ran binding protein 9(RanBPM) binds IFN-λR1 in the IFN-λsignaling pathway  

作　　者：Junwen Zhang XiaojieCong Jiajie Zhaoqiao Xia Yang Meng Li Hong Chen Ruifang Mi Guishan Jin Fusheng Liu Bing-Ren Huang 

机构地区：[1]National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China [2]Brain Tumor Research Center, Beijing Neurosurgical Institute, Beijing Tiantan Hospital Affiliated to Capital Medical University, Beijing 100050, China [3]Beijing Laboratory of Biomedical Materials, Beijing 100050, China

出　　处：《Science China(Life Sciences)》2017年第9期1030-1039,共10页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(81302186,81372354,81672478);the Beijing Natural Science Foundation(7151002);the Beijing Laboratory of Biomedical Materials Foundation,the Beijing Neurosurgical Institute Youth Programme(2014003,2016003);the Beijing Municipal Administration of Hospitals' Youth Programme(QML20150505)

摘　　要：Like the type I interferons(IFNs),the recently discovered cytokine IFN-λ displays antiviral,antiproliferative,and proapoptotic activities,mediated by a heterodimeric IFN-λ receptor complex composed of a unique IFN-λR1 chain and the IL-10R2 chain.However,the molecular mechanism of the IFN-λ-regulated pathway remains unclear.In this study,we newly identified RAN-binding protein M(RanBPM) as a binding partner of IFN-λR1.The interaction between RanBPM and IFN-λRl was identified with a glutathione S-transferase pull-down assay and coimmunoprecipitation experiments.IFN-λ1 stimulates this interaction and affects the cellular distribution of RanBPM.However,the interaction between RanBPM and IFN-λR1 does not correlate with their conserved TRAF6-binding sites.Furthermore,we also found that RanBPM is a scaffolding protein with a modulatory function that regulates the activities of IFN-stimulated response elements.Therefore,RanBPM plays a novel role in the IFN-λ-regulated signaling pathway.Like the type I interferons（IFNs）,the recently discovered cytokine IFN-λ displays antiviral,antiproliferative,and proapoptotic activities,mediated by a heterodimeric IFN-λ receptor complex composed of a unique IFN-λR1 chain and the IL-10R2 chain.However,the molecular mechanism of the IFN-λ-regulated pathway remains unclear.In this study,we newly identified RAN-binding protein M（RanBPM） as a binding partner of IFN-λR1.The interaction between RanBPM and IFN-λRl was identified with a glutathione S-transferase pull-down assay and coimmunoprecipitation experiments.IFN-λ1 stimulates this interaction and affects the cellular distribution of RanBPM.However,the interaction between RanBPM and IFN-λR1 does not correlate with their conserved TRAF6-binding sites.Furthermore,we also found that RanBPM is a scaffolding protein with a modulatory function that regulates the activities of IFN-stimulated response elements.Therefore,RanBPM plays a novel role in the IFN-λ-regulated signaling pathway.

关 键 词：IFN-λ IFN-λR1 INTERACTION RANBPM 

分 类 号：R346[医药卫生—基础医学]