一类酸响应、信号触发探针的生物成像应用  

Bio-imaging with a group of acid-responsive fluorescence-on probes

作　　者：刘剑[1] 薛钟慰[1] 赵虎[1] 韩家淮[2] 韩守法[1] 

机构地区：[1]厦门大学化学化工学院化学生物学系固体表面物理化学国家重点实验室谱学分析与仪器教育部重点实验室福建省化学生物学重点实验室,厦门361102 [2]厦门大学生命科学学院细胞应激生物学国家重点实验室细胞信号网络协同创新中心,厦门361102

出　　处：《中国科学：化学》2017年第8期973-985,共13页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21602185,21572189);福建省自然科学基金(编号:2016J05047);中央高校基本科研业务费专项资金(编号20720150047,20720160052)资助项目

摘　　要：水作为所有细胞和组织的重要组成部分,其pH和诸多生理活动密切相关.荧光分析具有可视化、高分辨率等特点,在生命体系的pH分析中得到广泛应用.近年来,pH触发荧光成像技术在生物医学领域逐渐得到应用.本文简要介绍了一类酸度触发荧光、含分子内螺环的罗丹明衍生物的设计与功能化,及其生物成像应用.pH is critical for diverse physiological activities. Disruption of pH homeostasis is pertinent to myriad pathological events.Particularly, the organelle-specific pH within mammalian cells is elaborately controlled and yet succumbs to pathological stress. Lysosomes are the major intracellular acidic compartments, and the lysosomal acidity is critical for diverse biological events. Aberrant lysosomal pH is manifested a broad spectrum of diseases ranging from inflammation to cancer metastasis, and to lysosomal storage diseases. As such, defining pH changes in specific pathological settings would be of use for biomedical investigations. Rhodamine-lactams, a group of nonfluorescent rhodamine derivatives featuring intramolecular spirolactam, are poised to proton triggered opening of the lactams to give fluorescent rhodamines, and has been widely used for lysosomal imaging in cell models. Herein we review the use of structurally modified rhodamine-lactams as acidity-reporting entities for bioimaging of distinct biological events,including host-pathogen interaction, cell death, autophagy, and mitochondrial depolarization in cells, and inflammation and tumors in mice models.

关 键 词：信号触发荧光成像 细胞器变化成像 糖缀合探针 炎癌活体成像 比率荧光成像 

分 类 号：O657.3[理学—分析化学]