近红外双发射荧光探针比率检测高/半胱氨酸  

A fluorescence probe with near-infrared dual-emission for the ratiometric determination of homocysteine and cysteine

作　　者：刘长辉[1,2,3] 李银辉[4] 齐风佩[2,3] 龙立平[2,3] 杨荣华[1] 

机构地区：[1]长沙理工大学化学与生物学院,长沙410004 [2]黑茶金花湖南省重点实验室 [3]湖南城市学院化学与环境工程学院,益阳413000 [4]湘潭大学化学学院,湘潭411105

出　　处：《中国科学：化学》2017年第8期1015-1021,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21135001,21405038,21575018,21545003,21675042);湖南省教育厅优秀青年项目(编号:15B043);黑茶金花湖南省重点实验室开放基金(编号:2016TP1022(HC6120))资助项目

摘　　要：合成的吲哚菁染料Cy5-A对高/半胱氨酸展现显著的荧光响应.高/半胱氨酸浓度的增加引发其荧光强度在660 nm处减弱,相应地在700 nm处增强,且700 nm与660 nm处的比率荧光变化与高胱氨酸(40.0~200.0 μM)及半胱氨酸(10.0~100.0 μM)浓度呈现良好的线性关系,最低检测限分别为13.2 μM与2.67 μM.通过核磁共振谱和质谱研究了Cy5-A与高/半胱氨酸的作用机制.研究表明,Cy5-A中的醛基与高/半胱氨酸发生环合反应,从而诱导产生近红外双发射光谱,探针响应不受其他干扰物的影响.因此,Cy5-A是一种简单高效的反应型荧光探针,可成功应用于血清样品中高/半胱氨酸的准确检测.In the paper, an indocyanine-based near infrared fluorescence probe （Cy5-A） was reported, which showed a remarkably fluorescent response to homocysteine or cysteine in the PBS buffer-acetonitrile solution （9：1, v/v） at room temperature. Furthermore, the increased concentration of homocysteine or cysteine caused the fluorescent intensity at 700 nm gradually enhanced, along with that at 660 nm decreased. Exhilaratingly, there was a ratio fluorescent response（F_（700）/F_（660）） to the concentration of homocysteine in the range of 40.0 to 200.0 μM with the limit of determination of 13.2 μM, or cysteine in the range of 10.0 to 100.0 μM with the limit of determination of 26.7 μM, respectively. The response mechanism of CyA-5 to homocysteine or cysteine was confirmed by ~1H NMR and MS, which was the cyclization reaction between the aldehyde group of CyA-5 with homocysteine or cysteine, inducing the near infrared dual-emission fluorescence signal. The fluorescent probe of CyA-5 exhibited good selectivity over other related amino acids and thiol-containing compounds. Therefore, the indocyanine based dye of CyA-5 was a simple and efficient reaction-basedfluorescence probe for the determination of homocysteine or cysteine in the serum samples with satisfactory results.

关 键 词：高/半胱氨酸 近红外 双发射 比率检测 荧光探针 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]