五甲川菁线粒体荧光探针:光稳定性与激发态寿命的关系  

Pentamethine cyanine located in mitochondria: relationship between photostability and excited state lifetime

作　　者：黄海桥[1] 龙飒然 李明乐 杜健军[1] 樊江莉[1] 彭孝军[1] 

机构地区：[1]大连理工大学精细化工国家重点实验室,大连116024

出　　处：《中国科学：化学》2017年第8期1035-1043,共9页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(编号:21421005;U1608222)资助项目

摘　　要：不同结构的五甲川菁荧光染料常被用于近红外荧光探针,其灵敏度高,但光稳定性不理想.本文合成了一系列中位引入电子给体对氨基苯或对羟基苯的五甲川菁染料,研究了取代基对光稳定性的影响.实验表明,具有氨基和羟基电子给体的染料具有较高的光稳定性.飞秒瞬态吸收实验证明,氨基降低了菁染料分子的激发态寿命,光稳定性与激发态寿命成负相关,同时染料能较好地定位于活细胞中的线粒体.这对设计高耐光牢度的新型菁染料探针具有重要意义.Pentamethine dyes are often used for near infrared fluorescent probes owing to the advantage of high sensitivity, however, their photostability is not so satisfying. In this paper, a series of pentamethine dyes which contain electron-donating groups like amino or hydroxyl groups were synthesized, and the effect of substituents on photostability and the application in cell imaging were also discussed. The results indicated that the photostability of fluorescent dyes which possess electron donors of amino and hydroxyl groups was high, and the lifetime of excited state of fluorescent probes were reduced after leading into the amino group proved by femtosecond transient absorption,respectively. Photostability is negatively correlated with the lifetime of the excited state and the fluorescent dyes are mainly localized in mitochondria of living cells. This research will be of importance for designing cyanine dyes with high photostability and good cellular stain properties.

关 键 词：菁染料 光稳定性 激发态寿命 生物成像 线粒体 

分 类 号：O657.3[理学—分析化学]