1株PRRSV类NADC30毒株的分离鉴定及序列分析  

Isolation,Identification and Sequence Analysis of a NADC30-like Strain of PRRSV

作　　者：崔丹丹[1] 王傲杰 王新港[1] 周峰[1] 彭志锋[1] 王川庆[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业科学》2017年第9期132-138,共7页Journal of Henan Agricultural Sciences

基　　金：河南省高校科技创新团队与支持计划资助项目(14IRTSHN015)

摘　　要：为了解猪繁殖与呼吸综合征病毒(PRRSV)流行株的变异情况,将PRRSV阳性病料处理后接种Marc-145细胞,成功分离到1株PRRSV毒株,将其命名为HENZXC-4,并对其全基因组序列进行测定和分析。结果表明,HENZXC-4为未发生重组的类NADC30毒株,其NSP2蛋白存在131个氨基酸不连续缺失,且其GP5蛋白存在1个氨基酸插入。To investigate the molecular variation characteristics of porcine reproductive and respiratory syndrome virus( PRRSV) in the field,a PRRSV isolate( HENXC-4) was isolated by inoculating the PRRSV positive samples into the Marc-145 cell layers. The whole genome was then sequenced and analyzed. The sequence analysis showed that HENXC-4 was NADC30-like PRRSV without any recombination phenomenon characterized by a discontinuous 131-amino-acid deletion in the NSP2 region.More importantly,it had a 1-amino-acid insertion in GP5,which was absent in other NADC30-like PRRSV strains.

关 键 词：猪繁殖与呼吸综合征病毒 分离鉴定 类NADC30株 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]