基因芯片早期检测中枢神经系统感染耐药基因  

Early detection of resistance genes in the central nervous system infection by gene chip technology

作　　者：王海波[1] 陈建[2] 张春秀[3] 管义祥[1] 张强[1] 

机构地区：[1]南通大学附属海安医院神经外科,南通22660 [2]南通大学附属医院神经外科 [3]生物芯片上海国家工程研究中心研发部

出　　处：《临床神经外科杂志》2017年第4期291-295,共5页Journal of Clinical Neurosurgery

基　　金：南通市社会发展科技计划资助项目(S10935)

摘　　要：目的探讨基因芯片技术在早期快速检测中枢神经系统感染耐药基因的临床意义。方法选取6种常见耐药基因的特异性DNA序列,设计并制作PCR引物及相应的探针,对60例中枢神经系统感染患者的脑脊液行基因芯片检测耐药基因及病原菌,将结果与传统脑脊液培养方法进行对比。结果传统脑脊液培养方法确诊中枢神经系统感染24例;基因芯片方法确诊中枢神经系统感染30例,并鉴定出菌种及耐药基因,另有30例未鉴定出菌种,但仍多数检出了耐药基因。结论基因芯片技术可以早期快速并灵敏的检测出中枢神经系统感染细菌的耐药基因,在早期明确诊断方面明显优于脑脊液细菌培养法。Objective To investigate the clinical significance of the gene chip technology for early detection of resistance genes in the central nervous system（ CNS） infection. Methods Six kinds of common resistance gene-specific DNA sequences were selected to design and produce the corresponding PCR primers and probes to detect resistant genes and pathogens in 60 cases of cerebrospinal fluid（ CSF） of patients with CNS infections,and cerebrospinal fluid culture results with the conventional method were for comparison. Results CSF confirmed the traditional culture methods were 24 cases of CNS infection,gene chip intracranial infections were diagnosed in 30 cases,intracranial infection pathogens and resistance genes identified,and another 30 cases of unidentified species,but most resistance genes still detected. Conclusion Gene chip technology can quickly and sensitive detect bacterial resistance genes in patients with CNS infections in early stage,and the technology is superior to cerebrospinal fluid bacterial culture for rapid clearly diagnosis.

关 键 词：基因芯片 中枢神经系统感染 耐药基因 

分 类 号：R446.5[医药卫生—诊断学]