更年滋肾汤合玉女酥对雌性去势大鼠下丘脑-垂体-性腺轴的影响  

Effect of Menopause Zishen Tang and Yunvshu on the Hypothalamus-pituitary-gonadal Axis of Female Gonadectomy Rats

作　　者：宋雨菊[1] 桑锋[2] 

机构地区：[1]南京中医药大学百草堂门诊部,江苏南京210023 [2]河南中医药大学第一附属医院艾滋病检测实验室,河南郑州450000

出　　处：《中外医疗》2017年第21期5-8,共4页China & Foreign Medical Treatment

摘　　要：目的观察更年滋肾汤合玉女酥对雌性去势大鼠性激素E2、FSH、LH、及神经递质5-HT、5-HTAA的影响,探讨药食合用对围绝经期综合征的疗效及作用机制。方法 2015年10月—2016年4月,选取上海斯莱克实验动物中心健康、12周龄、雌性未孕SD大鼠60只,随机分为正常对照组、模型组、中药(更年滋肾汤)组、食疗(玉女酥)组、药食合用组,每组12只。给药(食)6周后,检测指标:下丘脑、垂体、子宫、胸腺、脾脏重量系数变化;血清中E2、FSH、LH的含量变化;下丘脑5-HT和5-HTAA的含量变化。结果 (1)脏器重量系数:下丘脑、垂体各组间差异无统计学意义;子宫模型组(62.19±11.4)明显低于对照组(149.60±8.23),药食合用组(100.35±9.29)显著高于模型组;胸腺模型组(89.36±7.48)明显低于对照组(121.67±12.01),药食合用组(115.72±12.57)显著高于模型组;脾脏模型组(314.48±38.54)明显低于对照组(357.57±35.87),药食合用组(350.57±29.76)显著高于模型组,差异有统计学意义(P<0.05)。(2)血清性激素水平:E2含量,模型组(9.46±1.05)pg/m L明显低于对照组(13.97±1.14)pg/m L,中药组(12.94±1.73)pg/m L、食疗组(11.03±2.05)pg/m L、药食合用组(13.15±2.17)pg/m L均高于模型组(P<0.05);FSH含量,模型组(7.46±1.05)m IU/m L明显高于对照组(3.26±0.17)m IU/m L,中药组(4.34±0.73)m IU/m L和药食合用组(3.15±2.17)m IU/m L明显低于模型组(P<0.05,P<0.01);LH含量,模型组(13.07±1.05)m IU/m L明显高于对照组(7.74±1.12)m IU/m L,中药组(9.86±1.52)m IU/m L和药食合用组(7.83±1.07)m IU/m L明显低于模型组(P<0.05,P<0.01)。(3)5-HT水平,模型组(0.742±0.067)μg/g明显高于对照组(0.228±0.034)μg/g,中药组(0.357±0.043)μg/g、药食合用组(0.235±2.28)μg/g明显低于模型组(P<0.05,P<0.01);5-HTAA水平,模型组(3.986±0.520)μg/g明显高于对照组(1.109±0.230)μg/g,中药组(1.553±0.310)μg/g、药食合用组(1.211±0.270)μg/g明显低于模型组(P<0.05,P<0.01)。结�Objective To observe the effect of menopause zishen tang and yunvshu on the sex hormones E2,FSH,LH and neurotransmitter 5-HT,5-HTAA of female gonadectomy rats and study the curative effect and function mechanism of medicine and food on the perimenopausal syndrome. Methods 60 cases of female un-pregnancy SD rats aged 12 weeks in the Shangh ai Slrc Experimental Animal Center from October 2015 to April 2016 were selected and randomly divided into the normal control group, model group, TCM group, food therapy group and combined medicine and food group with 12 cases in each, after 6-week administration, the changes of weight of the hypothalamus, pituitary, uterus, thymus and spleen coefficient, changes of serum E2,FSH,LH contents and changes of contents of hypothalamus 5-HT and 5-HTAA were tested.Results The viscera weight coefficient: there were no obvious differences in the hypothalamus pituitary between groups, and the index in the uterus model group was obviously lower than that in the control group [(62.19±11.4) vs(149.60±8.23)],and the index in the combined group was obviously higher than that in the model group, which was(100.35±9.29), and the index in the thymus model group was obviously lower than that in the control group[(89.36±7.48) vs(121.67±12.01)], and the index in the combined group was(115.72±12.57), which was obviously higher than that in the model group, and the spleen model group was obviously lower than that in the control group, [(314.48±38.54) vs(357.57±35.87)], and the combined group was(350.57±29.76), which was obviously higher than that in the model group, and the differences were statistically significant(P<0.05), and the serum sex hormone level: E2 content in the model group was obviously lower than that in the control group [(9.46±1.05)pg/m L vs(13.97±1.14)pg/m L], and the index in the TCM group, food therapy group and combined group were respectively(12.94±1.73)pg/m L,(11.03±2.05)pg/m L and(13.15±2.17)pg/m L, which was higher than that in the model group(P<0.05), and the FSH

关 键 词：围绝经期综合征 下丘脑-垂体-性腺轴 更年滋肾汤合玉女酥 

分 类 号：R4[医药卫生—临床医学]