机构地区:[1]Grassland and Livestock Engineering Technology Research Center,Ningxia Academy of Agriculture and Forestry Sciences [2]Ningxia Tan Sheep Breeding Farm
出 处:《Animal Husbandry and Feed Science》2017年第4期209-212,共4页动物与饲料科学(英文版)
基 金:Supported by Agricultural Breeding Special Project of Ningxia Autonomous Region(2013NYYZ0404)
摘 要:[ Objective] The paper was to study the differential expression of growth hormone (GH) gene in different ages and different tissues of Tan sheep. [ Method] Using real-time fluorescent quantitative PCR (RT-PCR) assay, specific primers were designed according to the GH gene sequence published on Gen- Bank. With the total RNA of Tan sheep tissue as the template, the expression levels of GH gene in pectoral muscle, leg muscle and longissimus dorsi muscle at dif- ferent ages were analyzed. [ Result] In three types of muscle tissues, the expression patterns of GH gene in different tissues at the same month of age were pectoral muscle 〉 leg muscle 〉 longissimus dorsi muscle. The expression levels of GH gene were positively correlated with live weight before slaughter, carcass weight and net meat weight, and had significant positive correlation only with net meat weight ( P 〈 0.05 ). [ Conclusion ] The content of GH gene in different tissues gradually increased with the increasing months of age, and there were extremely significant differences in expression level of GH gene among different months of age or different sites (P〈0. 01).[ Objective] The paper was to study the differential expression of growth hormone (GH) gene in different ages and different tissues of Tan sheep. [ Method] Using real-time fluorescent quantitative PCR (RT-PCR) assay, specific primers were designed according to the GH gene sequence published on Gen- Bank. With the total RNA of Tan sheep tissue as the template, the expression levels of GH gene in pectoral muscle, leg muscle and longissimus dorsi muscle at dif- ferent ages were analyzed. [ Result] In three types of muscle tissues, the expression patterns of GH gene in different tissues at the same month of age were pectoral muscle 〉 leg muscle 〉 longissimus dorsi muscle. The expression levels of GH gene were positively correlated with live weight before slaughter, carcass weight and net meat weight, and had significant positive correlation only with net meat weight ( P 〈 0.05 ). [ Conclusion ] The content of GH gene in different tissues gradually increased with the increasing months of age, and there were extremely significant differences in expression level of GH gene among different months of age or different sites (P〈0. 01).
关 键 词:Tan sheep GH gene Real-time fluorescent quantitative PCR Expression pattern Correlation analysis
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