舌草总黄酮诱导人胃癌细胞凋亡及其作用机制初探  

Effects and Mechanisms of Total Flavones of Oldenlandia Diffusa Willd on Inducing Apoptosis of Human Gastric Carcinoma Cells

作　　者：高小民 张煦[1] 

机构地区：[1]兰州大学基础医学院病理研究所,甘肃兰州730000

出　　处：《亚太传统医药》2017年第17期13-15,共3页Asia-Pacific Traditional Medicine

摘　　要：目的:研究白花蛇舌草总黄酮(FOD)对人体分化胃腺癌BGC-823细胞增殖、凋亡的影响及其可能的作用机制。方法:MTT法检测FOD对BGC-823细胞增殖的影响;流式细胞术测定细胞周期与凋亡的变化;Western-blot法评价埃茲蛋白(Ezrin)、细胞间黏附分子1(ICAM-1)的表达情况。结果:FOD分别作用BGC-823细胞24h、48h后半数抑制浓度(IC50)依次为12.58、8.43μg/mL;阻滞细胞于G0/G1期(P<0.05)并诱导细胞凋亡(P<0.05);活化的Ezrin表达降低、ICAM-1表达升高。结论:FOD呈时间-剂量依赖性抑制BGC-823细胞增殖,阻滞细胞于G0/G1期,并诱导其凋亡,其诱导凋亡的机制可能与下调Ezrin、上调ICAM-1的表达有关。Objective：To invesgate the effects and possible molecular mechanisms of FOD on inducing apoptosis and inhibiting pro- liferation of human gastric carcinoma BGC-823 cells. Methods： The MTT assay was used to detect the antiproliferative effect of FOD on BGC-823 cells. Flow cytometry was applied to determine the cell cycle and apoptosis of BGC-823 cells. The expression level of ICAM-1 and Ezrin was evaluated by Western-Blot. Results：after dealed with by FOD for 24 and 48 h, the ICS0 of BGC-823 cells was 12.58 and 8.43 μg/mL respectively. The cell cycle of BGC-823 cells was blockaded in G0/G1 phase at the same time the apopto- sis rate was remarkably increased compared with that in control group （P〈0.05）. The expression of ICAM-1 raised and Ezrin de- creased. Conclusion ： FOD could be in a dose and time-dependent manner inhibit the proliferation and induce the apoptosis of BGC- 823 cells , It could significantly arrest cell cycle of BGC-823 cells at G0/G1 phase. The low expression of Ezrin and high expression of ICAM-1 may contribute to the apoptosis mechanism.

关 键 词：白花蛇舌草总黄酮(FOD) 周期阻滞 诱导凋亡 EZRIN and ICAM-1 

分 类 号：R285[医药卫生—中药学]