麒麟丸对少弱精子症模型雄性大鼠生殖功能的保护作用研究  

Protective effect of Qilin Pills on the reproductive function of oligoasthenospermia rats

作　　者：张开舒[1,2] 周芳[1,2] 安琪[2] 贾艳飞[1,2] 傅龙龙[1,2] 卢文红[2] 梁小薇[2] 商学军[3] 谷翊群[1,2] 

机构地区：[1]北京协和医学院研究生院,北京100730 [2]国家卫生和计划生育委员会男性生殖健康重点实验室,国家卫生计生委科学技术研究所/世界卫生组织人类生殖合作研究中心,北京100081 [3]南方医科大学附属金陵医院/南京军区南京总医院男科,江苏南京210002

出　　处：《中华男科学杂志》2017年第9期821-827,共7页National Journal of Andrology

摘　　要：目的:研究麒麟丸对雷公藤多苷所致少弱精子症模型雄性大鼠的生殖功能保护作用。方法:取28只雄性SD大鼠,随机分成正常对照组、模型组、麒麟丸低、高剂量组,除正常对照组外,其他各组灌服雷公藤多苷40 mg/(kg·d),同时麒麟丸给药组同日给予不同剂量麒麟丸保护,低剂量组给予麒麟丸1.62 g/(kg·d),高剂量组给予麒麟丸3.24 g/(kg·d)。连续给药30 d后,处死大鼠,称重大鼠睾丸,计算睾丸脏器指数,检测附睾精子浓度、活动率,检测生殖激素水平,评估抗氧化指标,HE切片观察大鼠睾丸组织形态学改变。结果:实验结束后,麒麟丸低、高剂量组精子浓度分别为(14.57±3.95)×10~6/ml、(39.71±11.31)×10~6/ml,精子活力为(3.71±1.11)%、(4.29±1.80)%皆较模型组(4.71±1.25)×10~6/ml、(0.57±0.53)%有明显提高(P<0.05);与模型组FSH(1.71±0.52)mIU/ml比较,麒麟丸低、高剂量组FSH下降,分别为(1.49±0.62)mIU/ml、(1.12±0.83)mIU/ml;fT水平较模型组升高(27.27±3.63)nmol/L、(32.80±2.51)nmol/L vs(22.81±2.75)nmol/L,SHBG水平升高(94.83±11.17)nmol/L、(88.05±9.21)nmol/L vs(56.74±8.29)nmol/L,差异皆有显著性(P<0.05),但T水平无明显变化(P>0.05)。与模型组比较,麒麟丸低、高剂量组SOD水平升高(277.14±15.84)U/ml、(299.60±20.83)U/ml vs(250.04±31.06)U/ml;ROS水平下降(397.61±62.71)IU/ml、(376.84±67.14)IU/ml vs(552.20±58.07)IU/ml(P<0.05)。与模型组比较,低、高剂量组HE染色显示麒麟丸能增加少弱精子症大鼠睾丸生精小管内各级生精细胞层次和数量。结论:麒麟丸能明显提高少弱精子症大鼠精子质量,改善生殖激素水平,减轻氧化应激损伤,改善大鼠睾丸组织形态,对生殖系统有较好的保护作用。Objective： To study the protective effect of Qilin Pills （QLP） on the reproductive function of rats with oligoastheno-spermia （OAS） induced by tripterygium glycosides. Methods： Twenty-eight nmle SD rats were randomly divided into a normal con- trol, an OAS model control, a low-dose QLP, and a high-dose QLP group of equal number. OAS models were made in the latter three groups by intragastrical administration of tripterygium glycosides at 40 mg per kg of the body weight per day, and meanwhile the animals in the low- and high-dose QLP groups were treated with QLP at 1.62 and 3.24 g per kg of the body weight per day, respectively, while those in the OAS model group with normal saline, all for 30 consecutive days. Then all the rats were executed for ohtaining the testis weight, testis viscera index, epididymal sperm concentration and motility, reproductive hormone levels, and antioxidation indexes and observation of the histomorphological changes of the testis tissue by HE staining. Results ： After 30 days of intervention, the low- and high-dose QLP groups, as compared with the OAS model controls, showed significantly improved epididymal sperm concentration （[14.57 ± 3.95] and [39.71 ± 11.31] vs [4.71 ± 1.25] xl06/ml, P 〈0.05） and motility （[3.71 ± 1.11] and [4.29 ± 1.80] vs [0.57 ± 0.531%, P 〈0.05）, increased levels of sex hormone binding globulin （SHBG） （[94.83 ± 11. 17] and [88.05 ± 9.21] vs [56.74 ± 8.29] nmol/L, P 〈0.05） and free testosterone （FT） （[27.27 ± 3.63] and [32.80 ± 2.51] vs [22.81 ± 2.75] nmol/L, P 〈0.05）, decreased level of follicle-stimulating hormone （FSH） （[1.49 ± 0.62] aml [1.12 ± 0.83] vs [ 1. 71 ± 0.52] mIU/ml, P 〈0.05）, but no significant change in the total testosterone （3T） level. Meanwhile, the level of superoxide dismutase （SOD） was markedly elevated in the low- and high-dose QLP groups in comparison with the OAS model control group （ [ 277.14 ± 15.84 ] and [299.60 ± 20.83 ] vs [ 250.04 ± 31.06 ] U/ml, P �

关 键 词：麒麟丸 少弱精子症 雷公藤多苷 生殖保护 氧化应激 

分 类 号：R285.5[医药卫生—中药学] R698.2[医药卫生—中医学]