异丙酚对幼鼠肝缺血再灌注时脑损伤的影响  

Effect of propofol on brain injury during hepatic ischemia-reperfusion in preadolescent mice

作　　者：石屹崴[1] 徐如彬[1] 王刚[1] 贾莉莉[1] 王菲[1] 喻文立[1] 

机构地区：[1]天津市第一中心医院麻醉科,300192

出　　处：《中华麻醉学杂志》2017年第7期825-827,共3页Chinese Journal of Anesthesiology

基　　金：天津市卫生计生委科技攻关项目（16KG101）

摘　　要：目的 评价异丙酚对幼鼠肝缺血再灌注时脑损伤的影响.方法 健康雄性C57BL/6小鼠48只,2周龄,体重6-9g,采用随机数字表法分为3组（n=16）：假手术组（S组）、肝缺血再灌注组（I/R组）和异丙酚组（P组）.采用夹闭肝左、中叶脉管共干制备肝缺血再灌注损伤模型.P组于切皮前30 min腹腔注射1％异丙酚30 mg/kg;S组和I/R组切皮前30 min腹腔注射等容量生理盐水.再灌注24 h时取血标本及脑组织,剥离海马.采用ELISA法测定海马TNF-α、IL-1β水平、血清TNF-α、IL-1β、S-100β蛋白和神经元特异性烯醇化酶（NSE）水平;光镜下观察海马病理学结果,采用TUNEL法确定海马细胞凋亡指数.结果 与S组比较,I/R组和P组血清及海马TNF-α、II-1β水平、血清S-100β蛋白和NSE水平和海马细胞凋亡指数升高（P〈0.01）,海马病理学损伤加重;与I/R组比较,P组血清及海马TNF-α、IL-1β水平、血清S-100β蛋白和NSE水平和海马细胞凋亡指数降低（P〈0.05）,海马病理学损伤减轻.结论 异丙酚可减轻幼鼠肝缺血再灌注诱发的脑损伤,机制可能与抑制全身和中枢炎症反应有关.Objective To evaluate the effect of propofol on brain injury during hepatic ischemiareperfusion （I/R） in preadolescent mice.Methods Forty-eight healthy male C57BL/6 mice.aged 2 weeks,weighing 6-9 g,were allocated to one of 3 groups （n=16 each） using a random nunber table：sham operation group （group S）,hepatic I/R group （group I/R） and propofol group （group P）.Hepatic I/R was produced by occlusion of the blood supply to the median and left lobes of the liver for 60 min followed by reperfusion in anesthetized mice.In group P,1% propofol 30 mg/kg was intraperitoneally injected at 30 min before skin incision,while the equal volume of normal saline was given instead in S and I/R groups.Blood samples were collected and brains were removed at 24 h of reperfusion,and hippocampi were then isolated.The levels of tumor necrosis factor-alpha （TNF-α） and interleukin-1beta （IL-1β） in hippocampi and levels of TNF-α,IL-1β,S-100β protein and neuron-specific enolase （NSE） in serum were determined by enzyme-linked immunosorbent assay.The pathological changes of hippocampi were examined with a light microscope,the apoptosis in hippocampal cells was measured using TUNEL,and the apoptotic index was calculated.Results Compared with group S,the TNF-α and IL-1β levels in serum and hippocampi,levels of S-100β protein and NSE in serum and apoptotic index were significantly increased （P〈0.01）,and the pathological changes of hippocampi were aggravated in I/R and P groups.Compared with group I/R,the TNF-α and IL-1β levels in serum and hippocampi,levels of S-100β protein and NSE in serum and apoptotic index were significantly decreased （P〈0.05）,and the pathological changes of hippocampi were attenuated in group P.Conclusion Propofol reduces brain injury induced by hepatic I/R,and the mechanism may be related to inhibition of systemic and central inflammatory responses of preadolescent mice.

关 键 词：二异丙酚 肝 再灌注损伤 脑 

分 类 号：R726.1[医药卫生—儿科]