Influence of White Spot Syndrome Virus Infection on Hepatopancreas Gene Expression of ‘Huanghai No.2' Shrimp(Fenneropenaeus chinensis)  

作　　者：MENG Xianhong SHI Xiaoli KONG Jie LUAN Sheng LUO Kun CAO Baoxiang LIU Ning LU Xia LI Xupeng DENG Kangyu CAO Jiawang ZHANG Yingxue ZHANG Hengheng 

机构地区：[1]Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences [2]Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology

出　　处：《Journal of Ocean University of China》2017年第5期863-872,共10页中国海洋大学学报（英文版）

基　　金：the Central Public-interest Scientific Institution Basal Research Fund, CAFS (No. 2016HY-ZD04);the National Natural Science Foundation of China (No. 31372523);the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02-03);the Taishan Scholar Program For Seed Industry, the Shandong Provincial Natural Science Foundation (No. ZR2014CQ001);the Special Fund for Postdoctoral Innovative Project of Shandong Province, and Central Public-Interest Scientific Institution Basal Research Fund of the Yellow Sea Fisheries Research Institute (Nos. 2060302013036 and 20603022015013)

摘　　要：To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus(WSSV) infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai No. 2'(Fenneropenaeus chinensis). A total of 59137 unigenes were designed onto a custom-made 60 K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early(48–96 h), peak(168–192 h) and late(264–288 h) infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes(1916 annotated) expressed at all three phases, and most of the annotated were either up-or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp's response to WSSV.To elucidate the molecular response of shrimp hepatopancreas to white spot syndrome virus（WSSV） infection, microarray was applied to investigate the differentially expressed genes in the hepatopancreas of ‘Huanghai No. 2＇（Fenneropenaeus chinensis）. A total of 59137 unigenes were designed onto a custom-made 60 K Agilent chip. After infection, the gene expression profiles in the hepatopancreas of the shrimp with a lower viral load at early（48–96 h）, peak（168–192 h） and late（264–288 h） infection phases were analyzed. Of 18704 differentially expressed genes, 6412 were annotated. In total, 5453 differentially expressed genes（1916 annotated） expressed at all three phases, and most of the annotated were either up-or down-regulated continuously. These genes function diversely in, for example, immune response, cytoskeletal system, signal transduction, stress resistance, protein synthesis and processing, metabolism among others. Some of the immune-related genes, including antilipopolysaccharide factor, Kazal-type proteinase inhibitor, C-type lectin and serine protease encoding genes, were up-regulated after WSSV infection. These genes have been reported to be involved in the anti-WSSV responses. The expression of genes related to the cytoskeletal system, including β-actin and myosin but without tubulin genes, were down-regulated after WSSV infection. Astakine was found for the first time in the WSSV-infected F. chinensis. To further confirm the expression of differentially expressed genes, quantitative real-time PCR was performed to test the expression of eight randomly selected genes and verified the reliability and accuracy of the microarray expression analysis. The data will provide valuable information to understanding the immune mechanism of shrimp＇s response to WSSV.

关 键 词：WSSV shrimp Spot differentially Huanghai No.2 Shrimp Infection proteinase custom tubulin 

分 类 号：S945.4[农业科学—水产养殖]