皮肤生理指标检测优选特应性皮炎润肤剂的实验研究  

Optimization of emollient formulation for treating atopic dermatitis by skin physiological index testing

作　　者：黄松根 杨西晓[3] 莫立乾[4] 周冼苡[1] 

机构地区：[1]南方医科大学第三附属医院皮肤科,广东广州510630 [2]南方医科大学,广东广州510515 [3]南方医科大学深圳医院药剂科,广东深圳518000 [4]南方医科大学南方医院药学部,广东广州510515

出　　处：《南方医科大学学报》2017年第7期967-974,共8页Journal of Southern Medical University

基　　金：天河区科技计划项目(201404KW048)

摘　　要：目的探讨以神经酰胺、透明质酸钠、丹皮酚、山茶籽油为主要成分研制润肤剂用于治疗特应性皮炎(AD)动物模型,并证实其有效性和安全性。方法按L9(34)正交实验表设计4种主要成分的配比,采用四因素三水平安排实验,将4~6周BALB/c小鼠制备的AD动物模型分为9组,分别涂抹9种主要成分浓度不一的润肤剂,通过检测治疗前及治疗后的皮肤含水量、经皮水分丢失(TEWL)筛选最优处方,并进行药效学及皮肤刺激性实验。结果通过正交表的极差分析以及方差分析得出神经酰胺对皮肤含水量影响最大,山茶籽油对TEWL影响最大,最优的处方为1号,主要成分的配比为D1E1F1G1;药效学实验显示高、中、低剂量组与空白对照组相比治疗后的皮肤含水量、pH值、TEWL值均明显改善,且P值均<0.05,与阳性对照组相比无统计学差异,P值均>0.05;皮肤刺激性试验结果为0分,无刺激性。结论该润肤剂能明显改善AD小鼠皮肤含水量、pH、TEWL,可用于修复AD小鼠的皮肤屏障,且无刺激性。Objective To optimize the formulation of an emollient for treatment of atopic dermatitis prepared using ceramide, sodium hyaluronate, paeonol, and camellia-seed oil. Methods The emollients with different ratios of the 4 components were designed according to the L9（34）orthogonal table with 4 factors and 3 levels. The efficacy of the prepared emollients was tested in 4-6 week-old BALB/c mouse models of atopic dermatitis to determine the optimal formulation of the emollient by evaluating skin water content, transepidermal water loss （TEWL）, pharmacodynamics and skin irritation. Results Range analysis of the orthogonal table and analysis of variance showed that ceramide and camellia seed oil contents had the greatest impact on the skin water content and TEWL, respectively, and the optimal composition of the emollient contained the 4 components at the ratios of D1E1F1G1. Pharmacodynamic experiments showed that at high, medium and low doses, the emollient with the optimal formulation significantly improved the skin water content, pH and TEWL in the mice （P〈0.05） with similar effects in the positive control group （P〉0.05） and a skin irritation test score of 0. Conclusion The emollient we prepared can significantly improve skin water content, pH and TEWL in the mouse model of atopic dermatitis without skin irritations.

关 键 词：特应性皮炎 润肤剂 皮肤屏障 皮肤生理指标 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]