基于FGFR1靶点分子虚拟对接筛选淫羊藿中抗骨质疏松的药效成分  

Screening medicinal components of anti-osteoporosis in Epimedii Herba based on FGFR1

作　　者：许梦习 耿诗涵 张译丹[1] 陈卓 杨珍[1] 张艳军[1] 庄朋伟[1] 

机构地区：[1]天津中医药大学中药学院天津市中药药理重点实验室,天津300193 [2]天津市药品检验研究院,天津300070

出　　处：《现代药物与临床》2017年第8期1403-1409,共7页Drugs & Clinic

基　　金：国家科技支撑计划项目(2011BAI07B08);长江学者和创新团队发展计划资助项目(IRT_14R41)

摘　　要：目的基于FGFR1蛋白靶点筛选淫羊藿中抗骨质疏松的药效成分。方法采用维甲酸灌胃14 d复制大鼠骨质疏松模型,给予淫羊藿提取物进行干预;采用Western blotting法检测各组大鼠股骨FGFR1蛋白的表达,用UPLC-Q-TOF-MS确认提取物中化学成分。基于FGFR1靶点进行分子虚拟对接筛选药效成分。结果淫羊藿能够改善大鼠骨质疏松症状,并提高FGFR1蛋白的表达。淫羊藿提取物中指认出23种化合物,其中淫羊藿苷、淫羊藿次苷I、箭藿苷C可与FGFR1可以有效对接。结论淫羊藿中淫羊藿苷、淫羊藿次苷I、箭藿苷C可能通过上调FGFR1发挥抗骨质疏松作用。Objective To Screen the medicinal components of anti-osteoporosis in Epimedii Herba based on FGFR1. Methods The osteoporosis of rats was induced by given retinoic acid intragastrically for 14 d, and were treated with Epimedii Herba extract. The expression of FGFR1 protein in the femur was detected by Western blotting, and the chemical constituents of the extract were confirmed by UPLC-Q-TOF-MS method. The effective components were screened based on the target of FGFR1 by molecular docking. Results Epimedii Herba had a significant function of anti-osteoporosis, and could increase the expression of FGFR1 protein in rats. Icariin, icarisid I and sagittatoside C were picked out among the 23 identified compounds in extract, and could be effectively docked with FGFR1. Conclusion Icariin, icarisid I and sagittatoside C may play an anti-osteoporotic effect by up-regulating FGFR1.

关 键 词：淫羊藿 骨质疏松 骨形成 淫羊藿苷 淫羊藿次苷I 箭藿苷C 分子对接 

分 类 号：R285.5[医药卫生—中药学] R965[医药卫生—中医学]