低硒模型大鼠对富硒大米和亚硒酸钠的生物利用效果比较  

Comparison of bioavailability between selenium-enriched rice and sodium selenite in low-selenium model rats

作　　者：郑婷婷[1] 王红平 孙卉[3] 王磊[3] 荣祖元 王娟秀 李亚丽[1] 邓远乐 阴文娅[1] ZHENG Ting - ting WANG Hong - ping SUN l-Iui WANG Lei RONG Zu - yuan WANG Juan - xiu LI Ya - li DENG Yuan - le YIN Wen - ya(Department of Nutrition, Food Hygiene and Toxicology, West China School of Public Health, Sichuan University, Chengdu , Sichuan 610041, china)

机构地区：[1]四川大学华西公共卫生学院(华西第四医院),四川成都610041 [2]四川省食品药品检验检测院,四川成都611731 [3]中国农业科学院生物技术研究所,北京100081 [4]成都市温江区妇幼保健院,四川成都611130

出　　处：《现代预防医学》2017年第17期3214-3219,共6页Modern Preventive Medicine

基　　金：公益性行业(农业)科研专项经费资助(201303106)

摘　　要：目的建立低硒大鼠模型,比较其对富硒大米和亚硒酸钠的生物利用效果。方法 81只3-4周龄雄性SD大鼠随机分为正常对照组和低硒模型组,建立低硒大鼠模型。将低硒大鼠随机均分为:低硒模型组,低、高水平亚硒酸钠组,低、高水平富硒大米组,饲喂相应饲料;8周后处死,测定全血及组织硒含量,血清、肝、肾中GSH-PX、SOD活性和TrxR、MDA含量。结果 (1)与低硒模型组相比,各富硒组全血及组织硒含量,血清、肝、肾GSH-PX、SOD活性及TrxR含量均升高,MDA含量降低(P<0.001)。(2)心、肝硒含量,肝TrxR含量:高水平富硒大米组高于其他富硒组(P<0.05);血清MDA含量:高水平富硒大米组低于其他富硒组(P<0.05);肝肾GSH-PX及肝SOD活性:2个高硒组分别高于2个低硒组(P<0.05);肝脏MDA含量:2个高硒组分别低于2个低硒组(P<0.05);肾脏硒含量、血清TrxR含量:高水平富硒大米组高于低水平富硒大米组。结论富硒大米生物利用效果优于亚硒酸钠,硒含量高的富硒大米有助于提升低硒模型大鼠抗氧化能力。Objective To compare the bioavailability of selenium - enriched rice and sodium selenite with the low - selenium rat model. Methods 81 male weaning SD rats were randomly divided into normal control group （NC） and low - selenium diet group to build low - selenium rat model. And then low - selenium rats were randomly divided into low - selenium model group （LS） ,low- level and high- level sodium selenite groups （LSS and HSS） and low- level and high -level selenium -enriched rice groups （LSR and HSR）. Rats in each group were fed with the corresponding feed. The selenium content,the GSH - PX and SOD activity and TrxR and MDA content in blood and tissue were measured after 8 weeks of intervention. Results （ 1 ） The selenium content,the GSH - PX and SOD activity and TrxR content in blood and tissue were higher in selenium - enriched groups than in LS,and the MDA content was lower in selenium -enriched groups than in LS （P 〈0. 001 ）. （2） The heart and liver selenium content and liver TrxR content were higher in HSR than in other selenium - enriched groups （ P 〈 0.05 ）, while the serum MDA content was just the opposite （P 〈0.05）. The GSH - PX and SOD activity in liver and GSH - PX activity in the kidney in HSR and HSS were respectively higher than those in LSR and LSS （P 〈 0.05） ,while the liver MDA content was just the opposite （ P 〈 0. 05 ）. And kidney selenium content and serum TrxR content were higher in HSR than in LSR. Conclusion The bioavailability of selenium - enriched rice is better than sodium selenite, and the high - level selenium - enriched rice can improve the antioxidant capacity of low - selenium model rats.

关 键 词：富硒大米 亚硒酸钠 GSH-PX SOD TrxR MDA 

分 类 号：R115[医药卫生—公共卫生与预防医学]