HIV新发感染者1型抗体蛋白印迹带型分析  

Blotting pattern of HIV-1 antibody protein in recent infections

作　　者：郑春梅 柯广强 ZHENG Chun - mei KE Guang - qiang(Affiliated Hospital of Guangdong Medical University Department of Infectioas Diseases, Zhanfiang, Guangdong 524001, China)

机构地区：[1]广东医科大学附属医院感染内科,广东湛江524001 [2]广东省湛江市疾病预防控制中心检验科,广东湛江524037

出　　处：《现代预防医学》2017年第17期3220-3222,共3页Modern Preventive Medicine

摘　　要：目的分析HIV-1抗体阳性新发感染者和长期感染者蛋白印迹带型变化,探讨它们的带型分布特征。方法对2014-2016年湛江市HIV-1抗体阳性的男男人群进行新发感染检测,确定新发感染组和长期感染组,对两组的蛋白印迹带型进行统计分析。结果膜蛋白gp41、gp120、gp160、酶蛋白p66和核心蛋白p24阳性率最高(总阳性率分别为94.7%、100.0%、100.0%、96.0%、100.0%),其次是酶蛋白p31、p51(总阳性率分别为84.0%、85.3%),核心蛋白p17(52.0%)和前体蛋白p55(24.0%)最低;新发感染者各带型阳性率总体上比长期感染者低,具体是膜蛋白gp120、gp160和核心蛋白p24始终保持100.0%阳性率,酶蛋白p66稍有下降(100.0%降到94.7%),而其它带型阳性率均有不同程度升高,且各带型在新发感染和长期感染中差异没有统计学意义(P>0.05);新发感染者缺失p17+p55(61.1%)比长期感染者(36.8%)要高很多(P=0.070)。结论带型p17+p55的缺失可能提示为新发感染者(P=0.070),待继续收集资料进一步阐明。Objective To analyze the change of the protein blotting of the recent and long - term infections with HIV - 1 antibody positive, and to learn about the features of type distribution. Methods New infection detection in gay men with HIV - 1 antibody positive was performed in Zhanjiang from 2014 to 2016. According to the results, these cases were classified into two groups,lncluding the recent infection group and the long - term infection group. The protein blotting pattern of the two groups was statistically analyzed. Results The membrane protein gp41 ,gp120,gp160 and core protein p24 showed the highest positive rates （94.7% ,100.0%, 100.0%, 100.0% ,respectively）. The enzyme protein p31 ,pS1 ,p66 were the next positive rates of 84.0% ,85.3% ,96.0%. And the positive rates of core protein p17 （52.0%） and the precursor protein p55 （24.0%） were the lowest. On the whole, the positive rates of protein pattern in long - term infections were higher than that in the recent infection. Specifically,the membrane protein gpl20,gpl6 and the core protein remained 100.0% positive rates. The membrane protein p66 had a slight decline （ from 100.0% to 94.7% ）. And the positive rates of the rest protein patterns all had increased at different degree. The protein patterns had no statistical difference （P 〉 0.05） between the recent infections and the on long- term infections. The missing rate of p17 ＋ p55 in the recent infection （61.1%） was much higher than that of the long -term infections（P = O. 070）, which was 36.8%. Conclusion The protein pattern which misses p17 ＋ p55 probably could mean recent infection （P = 0. 070）. But the further confirmation needs more research.

关 键 词：HIV-1型抗体 蛋白免疫印迹法 WB带型 新发感染者 

分 类 号：R113[医药卫生—公共卫生与预防医学]