二烯丙基二硫对IL-1β诱导SD大鼠关节软骨细胞的影响  

Effects of Diallyl Disulfide on SD Rat Articular Cartilage Cell Induced by IL-1β

作　　者：王宏强[1,2,3] 仇成凤[2,3] 邓紫薇[2,3] 史志华[2] 李峰[4] 谭力铭[1,2,3] 

机构地区：[1]湖南医药学院 [2]怀化市第一人民医院临床药学研究室 [3]怀化市第一人民医院循证医学与临床研究中心 [4]怀化市第一人民医院骨科,怀化418000

出　　处：《天然产物研究与开发》2017年第9期1498-1503,共6页Natural Product Research and Development

基　　金：湖南省教育厅科研计划(14C0915);湖南省中医药科研计划(201585);湖南省卫生科研计划(B2015-181)

摘　　要：观察二烯丙基二硫(DADS)对IL-1β诱导SD大鼠关节软骨细胞的影响,除空白对照组外,其余各组加入IL-1β(10 ng/m L)诱导24 h后换DADS处理,通过CCK8实验、平板克隆实验检测增殖能力,Western blot实验检测MMP13、TIMP1和CollagenⅡ蛋白表达水平。结果发现:12.5和25μmol/L DADS对IL-1β诱导SD大鼠关节软骨细胞有增殖作用(P<0.05),50和100μmol/L DADS对IL-1β诱导SD大鼠关节软骨细胞有抑制作用(P<0.05);12.5和25μmol/L DADS显著增加IL-1β诱导SD大鼠关节软骨细胞克隆集落形成能力(P<0.05),50μmol/L DADS显著抑制IL-1β诱导SD大鼠关节软骨细胞克隆集落形成能力(P<0.05);DADS能显著抑制MMP13的表达,增加TIMP1和CollagenⅡ的表达。以上结果提示DADS对IL-1β诱导SD大鼠关节软骨细胞具有双向调节作用,25μmol/L DADS保护关节软骨细胞的分子机制可能与DADS显著抑制MMP13的表达,增加TIMP1和CollagenⅡ的表达相关。To explore the effect of diallyl disulfide （DADS） on articular cartilage cell induced by IL-1β in SD rats. The growth inhibition of articular cartilage cell induced by IL-1β in SD rats following DADS treatment was measured by CCK8 assay and plate colony formation assay; The protein expression of M M P 1 3 , T I M P 1 and Collagen II were deter-mined using Western blot. DADS （12. 5 and 25 jjimol/L） significantly increased the cell proliferation and high colony formation capacity; DADS （50 and 100 jjimol/L） significantly suppressed the cell proliferation and lowered colony for-mation capacity （ P 〈 0. 05 ） ; Western blot demonstrated DADS markedly down-regulated the expression of MMP-1β , and increased the expression of Collagen II and TIMP1 in the cells. These results indicated that DADS had two-way regula-tion effect on SD rat articular cartilage cell induced by IL-1β,DADS （25 jjimol/L） had a protective effect on SD rat ar-ticular cartilage cell induced by IL-1/3,DADS （50 and 100 jjimol/L） can inhibit articular cartilage cell by IL-1β in SD rats,the protection molecular mechanism was possibly related to down-regulation of the expression of MMP-1β by DADS, and increased the expression of Collagen II and TIMP1 on SD rat articular cartilage cell with osteoarthritis.

关 键 词：二烯丙基二硫 IL-1Β SD大鼠关节软骨细胞 双向调节 

分 类 号：R285.5[医药卫生—中药学]