化瘀通络中药不同配伍对糖尿病肾病大鼠肾脏保护作用及对肾小球P-cadherin表达的影响  

Protective Effect of Different Combinations of Chinese Recipe of Stasis-removing-and-Collaterals-dredging on the Kidney of Diabetic Nephropathy Ratsand Its Effect onthe Expression of P-cadherin in the Glomerular

作　　者：霍贝贝[1] 白璐[1] 张肖[2] 陈志强[1,2] 郭倩[1] 徐晶[3] 

机构地区：[1]河北医科大学 [2]河北省中医院,石家庄050017 [3]河北中医学院,石家庄050200

出　　处：《天然产物研究与开发》2017年第9期1558-1562,共5页Natural Product Research and Development

基　　金：国家自然科学基金(81373804;81173419)

摘　　要：为观察化瘀通络中药不同配伍对糖尿病肾病大鼠肾脏的保护作用及对肾小球足细胞裂孔膜蛋白P-cadherin表达的影响,将70只雄性SD大鼠随机分为正常组和模型组、化瘀通络组、厄贝沙坦组、化瘀组和通络组,灌胃干预16周。检测大鼠的体重、肾重、肾脏指数、24 h尿蛋白定量,分别采用实时定量PCR和蛋白印迹法检测P-cadherin的表达。结果显示,与模型组比较,化瘀通络组大鼠的肾脏指数、24 h尿蛋白定量明显下降(P<0.01),体重增加、P-cadherin mRNA和蛋白表达明显上调(P<0.01)。提示化瘀通络中药可能通过上调P-cadherin mRNA和蛋白的表达,减少尿蛋白,延缓糖尿病肾病病程进展。To observe the protective effect of different combinations of Chinese recipe of stasis-removing-and-collaterals- dredging on the kidney of diabetic nephropathy （DN） rats and its effect on the expression of slit diaphragm protein P- cadherin in the podocyte,70 male Sprague Dawleg rats were randomly divided into control group,model group,stasis-re- moving-and-collaterals-dredging group,irbesartan group,stasis-removing group and collaterals-dredging group,and differ-ent medicines were intragastrically fed once a day for 16 weeks. Body weight, kidney weight, kidney index and 24 h uri-nary total protein were tested at the end of week 16. The expression of P-cadherin was detected by Real-time PCR,West- ern blot,respectively. The results showed that compared with the model group,kidney index,24 h urinary total protein of stasis-removing-and-collaterals-dredging group were significantly decreased （P 〈0. 01 ） ,body weight and the mRNA and protein expression of P-cadherin were significantly increased （P 〈0. 01 ）. It suggested that Chinese medicines of stasis- removing-and-collaterals-dredging may increase the mRNA and protein expression of P-cadherin to reduce urinary pro-tein and delay the progression of DN.

关 键 词：糖尿病肾病 化瘀通络 P-cadWm 裂孔膜蛋白 

分 类 号：R2-031[医药卫生—中西医结合]