大雁、斑头雁和天鹅源沙门菌的耐药性分析  

Analysis of Antimicrobial Resistance among SalmonellaIsolated from Geese,Bar-headed Geese and Swans

作　　者：葛润洲 罗薇[1] 杨怀珍[1] 孔雪英[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041

出　　处：《动物医学进展》2017年第9期49-54,共6页Progress In Veterinary Medicine

基　　金：四川省科技厅应用基础研究项目(2013JY0045)

摘　　要：研究大雁、斑头雁和天鹅源沙门菌的耐药性及其耐药基因的携带情况。采用K-B纸片法对33株大雁、斑头雁和天鹅源沙门菌进行20种抗菌药物敏感性测定,应用PCR对12种耐药基因的携带情况进行检测。结果表明,33株沙门菌分离株对阿莫西林、萘啶酸耐药性最高,分别为93.94%、87.88%,对氨苄西林(54.55%)、四环素(48.49%)、多西环素(48.49%)、卡那霉素(60.61%)、庆大霉素(48.49%)、链霉素(66.67%)耐药性比较严重,对氧氟沙星、培氟沙星、诺氟沙星敏感。共检测到9种耐药基因,其中blaCMY-2阳性率为87.88%,qnr、aac(6′)-Ib-cr阳性率分别为17.24%、86.21%,tetA、tetB阳性率分别为45%、20%,aph(3)Ⅱ、aac(3)Ⅱ、aadA1阳性率分别为100%、10%、20%,sul2阳性率为100%,未检出blaTEM-1、tetC、tetG基因。说明大雁、斑头雁和天鹅源沙门菌严重耐药,且携带多种耐药基因。The objective of this study was to investigate the antimicrobial resistance and the resistance genes in 33 pathogenic Salmonellastrains isolated from geese,bar-headed geese and swans with the Kirby-Bauer method to detect antimicrobial resistance and PCR detection for 12 antimicrobial resistant genes.The results indicated that the resistant rate to amoxicillin was 93.94%,nalidixic acid 87.88%,followed by streptomycin 66.67%,kanamycin 60.61%,ampicillin 54.55%,tetracycline 48.49%,doxycycline 48.49%,gentamicin 48.49%,and with no resistance to ofloxacin,pefloxacinand norfloxacin.A total of 12 kinds of resistant genes were amplified with the percentage of blaCMY-2 87.88%,qnr 17.24%,aac(6′)-Ib-cr86.21%,tetA 45%,tetB 20%,aph(3)Ⅱ 100%,aac(3)Ⅱ 10%,aadA120%,sul2 100%,and tetC,tetG and blaTEM-1were not detected.These results showed that the Salmonellaisolated from geese,bar-headed geese and swans were seriously resistant to antimicrobials.

关 键 词：沙门菌 耐药性 耐药基因 

分 类 号：S852.612[农业科学—基础兽医学]