特异性干扰survivin基因表达对人舌癌Tca8113细胞体外增殖和侵袭性的影响  

Influence of specific siRNA silencing survivin gene on proliferation and invasion of human tongue cancer Tca8113 cells in vitro

作　　者：母润红[1] 马方[1] 刘永超[1] 王湘茗[1] 

机构地区：[1]北华大学基础医学院,吉林132011

出　　处：《中国免疫学杂志》2017年第9期1310-1314,共5页Chinese Journal of Immunology

基　　金：吉林省教育厅项目(No.2014第202);江苏省检验医学重点实验室开放课题资助项目(No.JSKLM-2014-017);吉林市科技局项目(No.201339052)

摘　　要：目的:探讨应用RNAi技术特异性沉默survivin基因表达对人舌癌细胞Tca8113增殖、凋亡、迁移的影响,阐明survivin在Tca8113细胞生物学行为中的作用。方法:取对数生长期的Tca8113细胞,分为干扰组、阴性对照组及空白对照组。采用RT-PCR和Western blot方法检测Tca8113细胞中survivin基因的mRNA及蛋白的表达水平;MTT法检测细胞的增殖状况;流式细胞术检测各组Tca8113细胞凋亡率;划痕实验检测Tca8113细胞的迁移状况。结果:RT-PCR显示舌癌Tca8113细胞中survivin的mRNA表达水平明显低于空白对照组(P<0.05),Western blot蛋白免疫印迹结果显示,与空白对照组比较,survivin蛋白表达水平减少(P<0.05)。survivin干扰组Tca8113细胞的增殖能力降低(P<0.05);细胞划痕实验发现转染Tca8113细胞48 h后干扰组细胞的迁移距离明显短于空白对照组(P<0.05)。结论:特异性沉默siRNA-survivin的表达可有效抑制舌癌Tca8113细胞的体外迁移能力,可能与survivin表达减少有关,有望成为口腔舌癌生物治疗的潜在靶点。Objective To investigate the effect of siRNA interfering survivin gene on proliferation of human tongue cancerTca8113 cells,and clarify the effect of survivin Tca8113 cells biomechanism. Methods： The Tca8113 cells at logarithmic growth phase were selected and divided into interference group,negative control group,and blank control group. The relative levels of survivin mRNA and survivin protein expression of 3 groups wee detected by RT-PCR and Western blot assay,the inhibitory rate of proliferation of Tca8113 cells was checked by MTT method,the apoptotic rate was assessed by flow cytometry（FCM）,the the migration ability of Tca8113 cells were detected by Wound healing assay. Results： Compared with control group, the survivin mRNA and protein expression was markedly down-regulated in Tca8113 cells following RNA interference treatment（ P 〈0. 0 5 ） , the cell proliferation were down- regulated in interference group （ P 〈0.05） ,the cell apoptotic rate were up-regulated in interference group （ P 〈0. 0 5 ） , the migration ability was significantly decreased in interference group（ P 〈 0 .0 5 ） . Conclusion： The expression of siRNA-survivin can significantly inhibit the invasion in tongue cancer Tca8113 cells,indicating it might be a potential biological therapeutic target for tongue cancer.[

关 键 词：SURVIVIN SIRNA 增殖 凋亡 迁移 

分 类 号：R392.1[医药卫生—免疫学]