苦味类中药成份藉苦味受体途径对气道炎症过程的遏制效应  

Containment effects of bitter taste native ingredient from traditional Chinese medicine on airway inflammation process via bitter taste receptor pathway

作　　者：刘美花[1] 周向东[1] 李琪[1] 刘峰[1] 王杰[1] 钟有清[1] 

机构地区：[1]海南医学院第一附属医院呼吸内科,海口570102

出　　处：《中国免疫学杂志》2017年第9期1331-1335,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81660010;81611530713);海南省自然科学基金(2016-8305);海南省教育厅科研课题(Hnky2016-35);海南医学院大学生创新课题

摘　　要：目的:探讨苦味受体(Bitter taste receptor,TAS2Rs)在苦味类中药组份治疗慢性气道炎症中的抗炎效应。方法:实验分为三组(n=8),即空白对照组、PM2.5组(雾化吸入PM2.5悬液复制大鼠慢性气道炎症模型)和PM2.5+柠檬苦素组(陈皮提取物干预),分别以ELISA、RT-PCR和Westem blot检测各组大鼠支气管/肺组织炎症因子及TAS2Rs mRNA和蛋白表达水平。结果:PM2.5刺激组炎症因子TNF-α和IL-13的mRNA和蛋白表达水平较对照组明显增高,TAS2R14的mRNA和蛋白表达变化不明显;柠檬苦素干预组炎症因子TNF-α和IL-13的mRNA和蛋白表达水平较PM2.5组降低,TAS2R14的mRNA和蛋白表达水平则明显增高。结论:苦味类中药组份吸入治疗可遏制慢性炎性气道的炎症因子产生及释放,并刺激肺部TAS2Rs的表达增加,故认为苦味类中药组份激活气道TAS2Rs可能是其发挥抗炎效应的重要机制。Objective To investigate the anti-inflammatory effects of bitter taste receptor （TAS2Rs） in the treatment of chronic airway inflammation by bitter Chinese medicine. Methods： The experiment was divided into three groups （n = 8 ） , namely blank control group, PM2. 5 group （the rat models of chronic airway inflammation were established by aerosolized PM2. 5 suspension ） and PM2. 5 ＋ limonin group （intervening with the extract from Tangerine peel）. The expression of mRNA and protein of inflammatory cytokines and TAS2Rs in bronchiaL/pulmonary tissue were detected by ELISA, RT-PCR and Western blot respectively. Results： The mRNA and protein expression levels of TNF-α and IL-13 in PM2. 5 stimulated group were significantly higher than those in control group,while the TAS2R14 were not significantly changed. The mRNA and protein expression levels of TNF-α and IL-13 in the limonin intervention group was lower than that in the PM2. 5 group,and the TAS2R14 were significantly increased. Conclusion： The production and release of inflammatory cytokines in the chronic inflammatory airway can be curbed by inhaling the components of bitter Chinese herbal medi-cine. And then,the TAS2Rs in the lungs can be activated by the bitter components to increase its expression. Therefore,it is considered that bitter Chinese herbal medicine to play the airway anti-inflammatory effect is achieved through the activation of the airway TAS2Rs.[

关 键 词：苦味受体 苦味中药 炎症因子 气道炎症 

分 类 号：R56[医药卫生—呼吸系统]