间隙性连接蛋白37对动脉粥样硬化中单核巨噬细胞电流密度的影响  

Impact of Cx37 on the current density of mononuclear macrophage in atherosclerosis

作　　者：郭素峡[1] 尤华彦[2] 杨颖[2] 羊镇宇[2] 孟照辉[3] 肖践明[3] 

机构地区：[1]云南大学博士后流动站,昆明医学博士650031 [2]无锡市人民医院心脏中心,无锡214023 [3]昆明医科大学第一附属医院心内科,昆明650031

出　　处：《医学研究生学报》2017年第9期937-942,共6页Journal of Medical Postgraduates

摘　　要：目的国内外关于间隙性连接蛋白37(Cx37)基因改变巨噬细胞内外的电流密度,使得动脉粥样硬化进一步发展的研究甚少。文中旨在观察Cx37对动脉粥样硬化中巨噬细胞电流密度的影响。方法选取30只Wistar大鼠,Cx37+(保留Cx37基因)、Cx37-(沉默Cx37基因)各15只。采用高脂饮食建立动脉粥样硬化模型;根据标本采集部位分为4组:Cx37+斑块组、Cx37-斑块组(取自斑块)及Cx37+血液组、Cx37-血液组(取自血液),用RT-PCR检测Cx37+大鼠不同部位Cx37的表达;分离Cx37+、Cx37-大鼠血液中及斑块处单核巨噬细胞并培养,用全细胞记录法测定电流密度并进行比较。结果 Cx37+大鼠斑块中单核巨噬细胞中Cx37的相对表达水平显著高于血液[(1.1±0.02)vs(0.6±0.03)],差异有统计学意义(P=0.010)。Cx37+、Cx37-大鼠均行能谱CT检测鼠颈动脉斑块检测,验证了动物模型的成功建立。80、120、160 ms时,Cx37+斑块组电流密度[(0.61±0.06)、(0.67±0.07)、(0.91±0.03)A/cm^2]显著高于Cx37+血液组[(0.49±0.02)、(0.61±0.03)、(0.67±0.02)A/cm^2]、Cx37-斑块组[(0.48±0.02)、(0.60±0.02)、(0.64±0.02)A/cm^2]、Cx37-血液组[(0.49±0.02)、(0.59±0.02)、(0.64±0.02)A/cm^2];200、240、320 ms时亦显著高于Cx37+血液组、Cx37-斑块组、Cx37-血液组(P<0.05)。结论 Cx37对动脉粥样硬化中单核巨噬细胞斑块处电流密度的影响比外周血液更为显著,可促使巨噬细胞继续活化,动脉粥样硬化进一步发展。Objective Little research has been done on how Cx37 changes the current density of mononuclear macrophage in atherosclerosis. The purpose of this study was to detect the effects of Cx37 on the current density of mononuclear macrophage in athero-sclerosis. Methods A total of 30 Wistar mice were randomly divided into Cx37＋ group and Cx37-group equally. The atherosclerosis model was constructed by a high-fat diet. According to different parts of sample collection,these two groups were subdivided into Cx37 ＋ plaque group, Cx37-plaque group, Cx37＋blood group and Cx37-blood group. RT-PCR was applied to detect the expression of Cx37 in differ-ent body parts. The mononuclear macrophages were cultured after be-ing separated from blood and plague in both groups. The current density of mononuclear macrophage was detected by the whole cell re-cording. Results The relative expression of Cx37 in Cx37 ＋ plaque group was higher than that in plaque group （ [ 1 .10±0.02 ]vs [0.60±0.03] ）. Energy Spectrum CT was used to detect the carotid artery plaque in both Cx37 ＋ and Cx37- groups,which verified the successful model construction. At 80,120 and 160ms, the current density in Cx37 ＋ plaque group （[ 0.61 ± 0.06] , [0 .6 7 ± 0 .0 7 ], [0.91±0.03] A/cm2） was significantly higher than those in Cx37 ＋ blood group（ [0.49±0.02] , [0.61±0.03] , [ 0.67±0.02] A/cm2） ,Cx37-plaque group（ [ 0.48±0.02] , [ 0.60±0.02] , [ 0.64±0.02] A/cm2 ） and Cx37-blood group （ [ 0.49±0.02] , [ 0.59±0.02], [0.64±0.02] A/cm2）. The same goes for those at 200, 240, 320 ms（ P〈0.05）. Conclusion Cx37 has more significant impact on the current density in the plaque of mononuclear macrophage than in the peripheral blood in promoting macrophages activation and ath-erosclerosis progress.

关 键 词：动脉粥样硬化 间隙性连接蛋白37 斑块 电流密度 单核巨噬细胞 

分 类 号：R543.5[医药卫生—心血管疾病]