长期使用邦亭所致的血浆纤维蛋白原降低机制的初步实验研究  

Preliminary experimental study on hypofibrinogenemia in the long-term administration of hemocoagulase

作　　者：苑林 钟伟国 邓勤勤 徐宁 

机构地区：[1]广州中医药大学第二附属医院/广东省中医院芳村医院检验科,广州510370

出　　处：《国际检验医学杂志》2017年第17期2401-2403,共3页International Journal of Laboratory Medicine

基　　金：广东省中医院综合标准化试点重点示范项目资助(YN2015BZ10)

摘　　要：目的研究邦亭体内外降低血浆纤维蛋白原(FIB)及对与FIB合成相关的细胞因子白介素-6(IL-6)表达水平的影响。方法体外实验随机选取50份健康体检者混合血浆充分混匀后,随机平均分为7组,加入不同水平稀释的邦亭注射液后置于37℃温箱孵育,每12h检测FIB水平;动物实验选取6周龄健康昆明小鼠80只,雌雄各半,随机分为阴性对照组、低剂量组、中剂量组、高剂量组,每组20只,雌雄各半。各组按剂量腹腔注射邦亭3周后处死,分别检测凝血因子Ⅴ、凝血因子Ⅷ、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、FIB和IL-6水平。结果邦亭在体外有很强的降低FIB的作用,并呈现出明显的时间和剂量依赖性;邦亭长时间小剂量使用,可降低小鼠体内的FIB水平,但对凝血因子Ⅴ、凝血因子Ⅷ、PT、凝血酶时间(TT)并无明显影响;与阴性对照组相比,低、中、高剂量组的FIB、IL-6水平均下降,差异有统计学意义(P<0.05);各组间FIB水平比较差异有统计学意义(P<0.05);中、高剂量组的APTT轻微延长,与阴性对照组比较差异有统计学意义(P<0.05)。结论邦亭体内外均有较强的降低FIB的作用,临床长期用药时应密切检测患者的FIB水平,邦亭体内不仅直接分解FIB,可能还影响FIB合成相关的细胞因子水平,其具体机制有待更进一步的研究。Objective To study the effect of the long-term administration of hemocoagulasein vitro and in vivo,whether it may cause hypofibrinogenemia and changes of cytokine interleukin-6(IL-6)expression level which related to fibrinogen synthesis.Methods Totally 50 healthy subjects pooled plasma was chose in vitro experiments,which was divided into 7groups.After that,added various of dilutions of injection hemocoagulase and incubated at 37 ℃,detected FIB concentration every 12 h.In vivo experiments,80 rats with six-week old were randomly divided into 4groups:negative control group,high-dose group,middle-dose group,lowdose group,After 3weeks administration,the serum level ofⅤfactor,Ⅷfactor,PT,activated partial thromboplastin time(APTT),FIB,IL-6was detected.Results Hemocoagulase in vitro had a strong role to reduce fibrin,and showed a significant dose-dependent and time-dependent;Hemocoagulase prolonged low-dose use might reduce the concentration of FIB in mice,but theⅤfactor,Ⅷfactor,PT,APTT,TT were not significantly affected.Compared with the negative control group,FIB and IL-6concentration decreased in high-dose group,middle-dose group,low-dose group and had statistically significant differences(P<0.05);The level of FIB among the groups had statistically significant differences(P<0.05).The APTT of the middle and high dose groups was slightly prolonged,which was significantly different from that of the negative control group(P<0.05).Conclusion Hemocoagulase has a strong effect to reduce the concentration of fibrin,when there is a long-term medication,fibrin concentration of the patient should be closely monitored,hemocoagulase not only directly decomposed fibrin,but also may affect the synthesis of IL-6,the specific mechanism needs further study.

关 键 词：邦亭 纤维蛋白原 白介素-6 活化部分凝血活酶时间 凝血酶原时间 

分 类 号：R446.11[医药卫生—诊断学]