水稻红米色素基因的初步定位  

Primary Mapping of the Pigment Gene in Red Brown Rice

作　　者：吴建[1] 余显权[1] WU Jian YU Xian-quan(College of Agriculture, Guizhou University, Guiyang, Guizhou 550025, Chin)

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《山地农业生物学报》2017年第3期75-77,94,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技重大专项(水稻种质改良创新及新品种选育与应用)(黔科合重大专项字[2013]6023号);贵州省科技项目(贵州地方优质红米种质资源挖掘及其品质性状的研究)(黔科合基础[2016]1044)

摘　　要：本文运用生产中常用的恢复系蜀恢498为父本,贵州大学水稻研究所培育的贵红一号为母本构建的F4群体为材料,对水稻红米色素基因进行定位。经过经典遗传学分析确定了本实验材料的红米色素是由一对主效基因控制,其它微效基因具有修饰作用。运用基因池法筛选出12对具有多态性的SSR引物,分别位于第2、第4、第5、第6、第7和第10染色体上。运用mapmaker 3.0等定位软件,共检测到2个QTL位点,位于RM258-RM228的qAN-10a和位于RM5414-RM252的qAN-4a。其中qAN-10a在锦屏和惠水2个地点均检测到,具有良好的遗传稳定性,且贡献率较大,认为其为主效QTL,可为后续的精细定位提供参考。In this study,the pigment gene in the red brown rice was mapped using F4 population by crossing of Shuhui 498（male） and Guihong 1（female）.After the classical genetics analysis,it was confirmed that the red brown rice pigment of this experiment material was controlled by a pair of main genes,while other micro-genes played a role of modification effect.Twelve pairs of SSR primers with polymorphism were creened by gene pool method and.Using BAS method,12 pairs of polymorphism markers were screened between the parents and the gene pool;and they were respectively located on the 2 nd,4 th,5 th,6 th,7 th and 10 th chromosomes.Using mapmaker 3.0 location software,two quantitative trait locus（QTL） locations were identified：qAN-10 a located between RM 258 and RM 228 and qAN-4 a located between RM 5414 and RM 252.qAN-10a had been detected on two sites of Jinping County and Huishu County,respectively.qAN-10 a has good genetic stability and big gentic contribution rate,and was considered as the main effect gene of QTL,thus,provides a references to subsequent mapping of this major QTL.

关 键 词：水稻 花色苷 QTL 

分 类 号：Q958.1[生物学—动物学] S443.3[农业科学—农业昆虫与害虫防治]