宫颈癌中结缔组织生长因子的表达及其相关因素分析  

Expression of connective tissue growth factor in cervical cancer tissues and its correlative factors

作　　者：连相尧[1] 郑小影[2] 郝云涛[3] 赵淑敏[2] 马卫军[2] 刘胜[2] 

机构地区：[1]承德医学院附属医院肿瘤内科,河北承德067000 [2]承德医学院基础医学院电镜室,河北承德067000 [3]承德医学院附属医院妇科,河北承德067000

出　　处：《中国临床药理学杂志》2017年第17期1641-1643,共3页The Chinese Journal of Clinical Pharmacology

基　　金：河北省卫计委医学科学研究重点课题计划基金资助项目(20150015);承德市科技局科学研究与发展计划基金资助项目(20122148;20142031)

摘　　要：目的探讨结缔组织生长因子(CTGF)在子宫颈癌组织中的表达水平及其与临床病理因素间的关系。方法用实时荧光定量聚合链式反应和免疫印迹法检测61例子宫颈癌癌组织(试验组)和对应癌旁正常宫颈组织(对照组)中CTGF mRNA及蛋白的表达水平,并进行多因素Logistic回归分析。结果对照组和试验组的CTGF mRNA表达量分别为(1.00±0.01)和(0.30±0.03),CTGF蛋白的表达量分别为(0.98±0.02)和(0.22±0.05),差异均有统计学意义(均P<0.01)。多因素Logistic回归分析结果显示:CTGF mRNA表达与宫颈癌的FIGO分期、淋巴结转移和分化程度呈负相关,CTGF蛋白表达与FIGO分期呈负相关。结论 CTGF在宫颈癌中低表达,可能作为抑癌因子在宫颈癌的发生发展中发挥作用。Objective To investigate the expression of connective tissue growth factor （CTGF） and their correlative factors in cervical cancer. Methods We quantified CTGF expression levels in 61 cervical cancer tissues（ treatment group ） and normal cervical tissues （control group ） around the tumor with real time polymerase chain reaction （PCR） assay and Western blot and analyzed correlative factors by logistic regression. Results The expression of CTGF mRNA in control group and treatment group were （1.00 ± 0.01）, （0.30 ± 0.03） respectively, and （0.98 ± 0.02）, （ 0. 22 ± 0. 05 ） in terms of the expression of CTGF protein, which had statistical significance （all P 〈 0. 01 ）. Multiple factor logistic regression showed that the expression of CTGF mRNA was negatively correlated with Federation International of Gynecology and Obstetrics （FIGO） staging, lymph node metastasis and differentiation of cervical cancer, and the expression of CTGF protein was negatively correlated with FIGO tumor suppressor gene staging. Conclusion CTGF may play a role as in the progression of cervical cancer.

关 键 词：宫颈癌 结缔组织生长因子 免疫印迹法 

分 类 号：R97[医药卫生—药品]