山东省鸡源致病性大肠杆菌O-抗原混合血清型分析  

Analysis of mixed O-antigen serotypes of E.coli isolated from chickens in Shandong province

作　　者：冯敏燕[1] 林树乾[1,2] 房玉波 李桂明[1] 杨世发[1] 赵增成[1] 黄中利[1] 傅剑[1,2] 商晶 宋敏训[1] 

机构地区：[1]山东省农业科学院家禽研究所,山东济南250023 [2]山东吴泰科技药业有限公司,山东济南250023 [3]山东省沂源县动物卫生监督所,山东沂源256100

出　　处：《中国兽医学报》2017年第9期1676-1679,共4页Chinese Journal of Veterinary Science

基　　金：山东省重点研发计划资助项目(2015GNC113004);山东省现代农业产业技术体系资助项目(SDAIT-13-011-01);山东省农业科学院农业科技创新工程资助项目(CXGC2016A10);防治家禽细菌性疾病新型中兽药制剂的开发及应用资助项目(201602085);山东省农业重大应用技术创新资助项目(鲁财农指[2016]36号)

摘　　要：采用抗原抗体凝集反应,用常见的18种O因子血清测定从临床上分离鉴定的182株大肠杆菌的O抗原血清型。结果显示,山东地区鸡源大肠杆菌的优势血清型为O24,O15,O2,O127,O1,O18,O78,占检测菌株的41.21%,同时出现大量混合血清型,占检测菌株的24.18%。这是国内首次报道检测到大量的大肠杆菌O因子混合血清型,印证了国外研究人员对O-AGC核苷酸序列研究的结果,有助于最终对O抗原血清型进行更加准确的分类以及大肠杆菌疫苗的研制。The O-antigen serotypes of 182 strains of E. coil,which were isolated and identified from chicken of different districts in Shandong province,were tested against 18 kinds of O factor serum by antigen-antibody agglutination reacting. The results shows the dominant serotypes of Esche- richia coli in Shandong province are O24, O15,02, O127, O1, O18 and 078 respectively, which ac- counts for 41.21% ,and displayed a lot of mixed O-antigen serotypes which accounts for 24.18% of tested strains. It is first reported that there are many mixed O-antigen serotypes of Escherichia coli in China,which are in accord with previous reports from foreign.

关 键 词：大肠杆菌 O-抗原 交叉反应 混合血清型 

分 类 号：S852.61[农业科学—基础兽医学]