PYY^(3-36)对LPS诱导的BV-2细胞中促炎酶类及促炎细胞因子的影响  

Effects of PYY^(3-36) on pro-inflammatory enzymes and pro-inflammatory cytokines in LPS-induced BV-2 cell lines

作　　者：曾亚龙[1] 阚兴池 吴艳成 孙建华[1] 冯文倩[1] 王玮[1] 付守鹏[1] 柳巨雄[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2017年第9期1736-1742,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31672509);吉林省科技发展计划资助项目(20170623029TC)

摘　　要：为明确酪酪肽(peptide YY,PYY^(3-36))对脂多糖(lipopolysaccharide,LPS)诱导的BV-2细胞中促炎细胞因子及促炎蛋白酶类的影响,本试验用不同浓度的PYY^(3-36)预处理后,再用LPS刺激BV-2细胞,利用MTT法检测PYY^(3-36)的细胞毒作用;分别用荧光定量RT-PCR和Western blot方法检测促炎细胞因子(IL-1β、IL-6和TNF-α)及促炎蛋白酶类(iNOS和COX-2)基因水平和蛋白水平表达变化;分别利用NO检测试剂盒和ELISA试剂盒检测iNOS和COX-2的主要产物NO和PGE2;利用PCR方法检测PYY^(3-36)受体表达情况。结果显示,PYY^(3-36)(10-9~10-11 mol/L)呈浓度依赖性抑制LPS在BV-2细胞中诱导的促炎细胞因子和促炎蛋白酶类的基因和蛋白水平,并能浓度依赖性抑制NO和PGE2。PCR结果表明BV-2细胞中PYY^(3-36)受体Y1、Y2、Y5和Y6表达明显,并且LPS刺激后Y2R受体表达增加。表明PYY^(3-36)在BV-2细胞中能抑制LPS诱导的促炎细胞因子和促炎蛋白酶类的表达以及促炎酶类产物的生成,这个过程可能涉及到其受体Y2。To investigate the effects of peptide yy3-36 （pyy3-36） on secretion of pro-inflammatory cytokines and pro-inflammatory enzymes in BV-2 cell lines. The BV-2 cells were treated with pyy3-36 at different concentration for 1 h prior the incubation of LPS. The levels of pro-inflamma- tory cytokines and pro-inflammatory enzymes were determined with real-time quantitative PCR and Western blot. The concentration of NO and PGE2 were detected by NO assay kit and ELISA Kit. PCR method was used to detect the expression of pyy3-36 receptor. The results showed that the production of pro-inflammatory cytokines, pro-inflammatory enzymes,NO and PGE2 in the LPS-induced BV-2 cells were suppressed by pyys 36 （10^-9-10^-11 mol/L） in a dose-depend manner. PCR results showed that the expression of pyy3-a6 receptor Y1, Y2, Y5 and Y6 was detected in BV- 2 cells,and the expression of Y2R was observed to significantly increase after LPS stimulation. These results suggest that pyy3-36 significantly reduces levels of pro-inflammatory cytokines, pro- inflammatory enzymes,NO and PGE2,and Y2R have an important role in this process.

关 键 词：PYY3-36 BV-2细胞 促炎细胞因子 促炎蛋白酶类 

分 类 号：S852.33[农业科学—基础兽医学]