阿替美唑对舒泰/噻啦嗪诱导大鼠丘脑和大脑皮质c-fos基因表达的影响  

Effect of atipamzole on telazol/xylazine-induced expression of c-fos in rat thalamencephal and cerebral cortex

作　　者：尹柏双[1,2] 高利[3] 石星星[3] 付莹[1] 沙万里[1] 王洪斌[3] 

机构地区：[1]吉林农业科技学院动物科技学院,吉林吉林市132101 [2]吉林农业科技学院预防兽医学吉林省重点实验室,吉林吉林市132101 [3]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医学报》2017年第9期1748-1752,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31302150;31272617;31001092)

摘　　要：24只SD大鼠被随机分成对照组、麻醉组和催醒组,对照组注射二甲基亚砜,麻醉组注射舒泰(13.81mg/kg)和噻啦嗪(5.21mg/kg),催醒组在给予麻醉剂10min后注射阿替美唑(0.522mg/kg),试验组大鼠分别于给药1h(即麻醉期)即刻,断头处死,冰面上分离丘脑和大脑皮质,利用免疫印记技术测定Fos蛋白表达量。结果显示,舒泰联合噻啦嗪注射后引起麻醉期大鼠丘脑和大脑皮质脑区Fos蛋白表达量显著高于对照组(P<0.01),阿替美唑注射后显著地抑制了舒泰联合噻啦嗪诱导大鼠丘脑和大脑皮质脑区Fos蛋白表达(P<0.01)。结果表明,阿替美唑可抑制舒泰联合噻啦嗪麻醉引起大鼠中枢脑区Fos蛋白的表达,对神经损伤起到一定的保护作用。The aim of the study was to assess whether the effect of atipamezole on talezol/xylazine induced express of c-fos in rat brain. Rats were injected with the mixture of 13.81 mg/kg telazol and 5.21 mg/kg xylazine,following 10 min later injected 0. 529 mg/kg atipamezole,and then the thalamencephal and cerebral cortex were removed at 1 h after injected. Protein level of Fos was measured in the brain tissue by Western blot. The results revealed that after telazol/xylazine ad- ministrated,the content of c-fos protein was increased significantly compared with the control group （P〈0.01）, atipamezole attenuated telazol/xylazine inducting c-fos expression in the thalam- encephal and cerebral cortex （P〈0.01）. The results indicated that atipamezole is able to inhibite telazol/xylazine-induced c-fos expression in the rat brain,playing a protective role of neuronal inju- ry.

关 键 词：阿替美唑 舒泰 噻啦嗪 大鼠 C-FOS 脑 

分 类 号：S859.7[农业科学—临床兽医学]