禽腺病毒4型感染病理组织学观察  

Pathohistological observation of avian adenovirus type 4 infection

作　　者：陈萍[1] 朱佳佳[1] 马晓勇[1] 张姗[1] 经美 崔元[1] 董世山[1,2] 陈立功[1,2] 刘聚祥[1,2] 孙继国[1,2] 袁万哲[1,2] 

机构地区：[1]河北农业大学动物医学院,河北保定071001 [2]河北省兽医生物技术工程技术研究中心,河北保定071001

出　　处：《中国兽医学报》2017年第9期1753-1755,共3页Chinese Journal of Veterinary Science

基　　金：河北省现代农业产业技术体系蛋鸡产业创新团队专项资金资助项目(HBCT2013090202);河北省高校百名优秀创新人才支持计划资助项目(Ⅲ)(SLRC2017039);2016年河北农业大学大学生创新创业训练计划项目;河北省大学生创新创业训练计划资助项目(201610086055)

摘　　要：将30只1日龄SPF鸡随机分为2组,其中攻毒组20只,对照组10只。攻毒组按每只0.2mL腹腔注射禽腺病毒4型(FAV-4)病毒液,对照组不做处理,隔离饲养,临床观察2周。攻毒后第3天攻毒组开始出现死亡,将死亡鸡解剖观察各器官大体病变,经聚合酶链式反应(PCR)检测为FAV-4后,采集心脏、肝脏、脾脏、肾脏、肺脏制作石蜡切片,观察病理组织学变化。结果显示,攻毒组死亡鸡只无明显临床症状而突然倒地死亡;主要剖检病变为心包积液、肝脏肿大,个别可出现脾脏肿大、肺脏淤血、肾脏肿大;病理组织学变化为肝脏、肺脏、肾脏淤血,细胞变性坏死;而对照组无任何异常症状和病理组织学变化。Thirty healthy 1-day-old SPF chicken were randomized into two groups,one for the infec- ted group（20 chickens） and the other for the control group（20 chickens）. The infected group was infected with 0.2 mL FAV-4 by intraperitoneal injection,while the control group was not treated. Clinical observation lasted for 2 weeks. Third days after the infected,the infected chichen began to die,the gross lesions of various organs were observed. After determined virulent FAV-4 by PCR, collected heart,liver, spleen, kidney and lung to make paraffin sections,observe the pathohistologi- cal changes. The results revealed, the infected group have no obvious clinical symptoms but sudden death ; the main pathohistology changes were pericardial effusion, hepatomegaly, the individual may be splenomegaly, lung congestion,renomegaly. The histopathological changes were hemorrhage, cell degeneration and necrosis in liver,lung,kidney. However, no symptoms and pathohistological changes were found in control group.

关 键 词：禽腺病毒4型 鸡 病理组织学 

分 类 号：S852.31[农业科学—基础兽医学] S852.65[农业科学—兽医学]