硒化低聚氨基多糖对小鼠急性毒性试验  

Acute toxicity of low molecular seleno-aminopolysaccharide to mice

作　　者：王晓波 闻正顺 张鹏[2] 曲有乐 许梓荣[3] 

机构地区：[1]浙江海洋大学食品与医药学院,浙江舟山316000 [2]浙江省海洋水产养殖研究所,浙江温州325005 [3]浙江大学动物科学学院,浙江杭州310058

出　　处：《中国兽医学报》2017年第9期1790-1796,共7页Chinese Journal of Veterinary Science

基　　金：浙江省自然科学基金资助项目(LY17C170001);国家自然科学基金资助项目(31301982);浙江海洋学院2012年科研计划资助项目(面上项目)(X12M05);浙江海洋学院校级重大资助项目(X12ZD08);浙江省舟山市科技计划资助项目(2016C41015)

摘　　要：研究硒化低聚氨基多糖(LSA)的安全性,采用寇氏法和食品安全国家标准(GB 15193.3-2014)进行了急性毒性评价,计算了LSA对ICR小鼠的半数致死量(LD50)和95%置信区间,并通过小鼠给药后体质量、死亡情况、器官指数、病理变化评价LSA对小鼠的急性毒性。结果显示:LSA对小鼠灌胃给药的LD50为2 884 mg/kg,其95%可信限为2 378~3 497mg/kg。与空白对照组相比,各组小鼠心脏、肝脏、脾脏、肾脏指数差异不显著(P>0.05),最大剂量(10 000mg/kg)组肺脏和胸腺指数差异显著(P<0.05)。LSA急性中毒死亡小鼠各组织均有出血状况,其中肺部淤血较为严重,小肠内容物暗红色,个别出现肠臌胀。结果表明,LSA属于低毒物质,毒性作用小,可以对其药效进行进一步研究。To study the acute toxicity of low molecular seleno-aminopolysaccharide （1.8 × 10^4 mg/kg se- lenium） in mice, the Karber method and national food safety standard （GB 15193. 3-2014） was used to calculate the median lethal dose （LD50） values and the 95% confidence limits for the acute toxicity study of the drug in mice. The results showed that the LD50 values of low molecular sele- no-aminopolysaceharide dispersion in mice by intraperitoneal injection were 2 284 mg/kg,and the 95% confidence limits were between 2 378 and 3 497 mg/kg. Compared with the blank control group,the difference of the cardiac, hepatic, spleen and kidney relative index in the others group was not significant（P〉0.05）, the difference of lung and thymus index was significantly （P〈0.05） in maximum dose （10 000 mg/kg） group. Various organs of dead mice were bleeding,inclu- ding serious lung congestion. The intestinal contents is dark red and some mice displayed intestinal bloat. The results indicate that low molecular seleno-aminopolysaecharide is a low toxic substance, and can be used to carry out a drug efficacy test.

关 键 词：硒化低聚氨基多糖 急性毒性 半数致死量(LD50) 寇氏法 

分 类 号：S856.9[农业科学—临床兽医学] S865.13[农业科学—兽医学]