金红茵芋离体快速繁殖技术的研究  

Research of in vitro rapid propagation technique of Skimmia japoniac 'rnbella'

作　　者：黄益基 吕秀立 付瑞 郭小芳 方腾 

机构地区：[1]上海市园林科学规划研究院,上海200232 [2]浙江省长兴县水口乡林业工作站,浙江长兴313108 [3]上海城市困难立地绿化工程技术研究中心,上海200232 [4]国家林木种质资源平台-上海子平台,上海200232 [5]德州世纪风园艺科技创新有限公司,山东德州253500 [6]嘉善县笠歌生态科技有限公司,浙江嘉善314113

出　　处：《江苏林业科技》2017年第4期8-12,17,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：上海市种业发展项目"特色蔬菜资源收集;特色良种筛选及标准化繁育"(沪农科种字(2015)第8号);上海市崇明区可持续发展科技创新行动计划"岩白菜种植技术集成与示范应用"(CK2017-50)

摘　　要：为了建立完善的金红茵芋快速繁殖技术体系,以带腋芽茎段为外植体,对其进行了离体培养。结果表明:初代培养基以MS+0.2 mg/L ZT+0.04 mg/L IBA+5.0 mg/L GA3较为适宜,外植体诱导率最高达85%;最佳增殖培养基是MS+1.0 mg/L ZT+0.5 mg/L KT+0.1 mg/L IBA,培养30 d后,增殖率达3.6,试管苗高度达2.0 cm;生根培养基以1/2MS+1.0 mg/L IBA效果为最佳,生根率达90%;适时移栽入泥炭+蛭石+珍珠岩+有机肥(体积比为6∶2∶1∶1)的混合基质中,成活率在80%以上。The nodal segments with auxiliary bud from Skimmia japoniac‘rnbella＇were used as explants for micro-propagation. After the explants were cultured on the MS media with 0. 2 mg/L ZT,0. 04 mg/L IBA and 5. 0 mg/L GA3 for 30days,the sprout induction percentage reached 85%. During the multiplication stage of 30 days,the sprouts cultured on the MS medium with 1. 0 mg/L ZT,0. 5 mg/L KT and 0. 1 mg/L IBA could get shoot multiplication of 3. 6,and height of 2. 0cm. In the consecutive rooting step,isolated shoots placed in 1/2 MS media containing 1. 0 mg/L IBA could get the rooting rate of 90%. The survival of plantlets was more than 80% for the appropriate time of transplant,with transplant substrate mixture（ containing peat soil,vermiculite,perlite and manure） fixed as a volume ratio of 6 ∶2 ∶1 ∶1.

关 键 词：金红茵芋 带腋芽茎段 外植体 诱导 增殖 生根 基质 

分 类 号：Q943.1[生物学—植物学] Q949.752.7