桃PpSnRK1β1和PpSnRK1β2在番茄中超表达对光合碳代谢的影响  

Effects of Over-expression of Peach PpSnRK1β1 and PpSnRK1β2 on Photosynthetic Carbohydrate Metabolism in Tomato

作　　者：王贵芳[1] 彭福田[2] 于雯[2] 赵永飞[2] 罗静静[2] 肖元松[2] 陈晓璐[2] 

机构地区：[1]山东省果树研究所,山东泰安271000 [2]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《园艺学报》2017年第8期1429-1438,共10页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31672099);国家现代农业产业技术体系建设专项资金项目(CARS-31-3-03);山东省“双一流”大学建设计划奖补资金项目(SYL2017YSTD10)

摘　　要：从毛桃[Prunus persica(Linn.)Batsch.]叶片中克隆了PpSnRK1蛋白激酶β亚基编码基因PpSnRK1β1和PpSnRK1β2,其c DNA全长分别为720 bp和867 bp。q RT-PCR组织表达分析表明,PpSnRK1β1在‘鲁星’桃叶片中的表达量最高,PpSnRK1β2在其果实中表达量最高,两者在其花中的表达量均较低。通过农杆菌介导转化番茄,获得超表达PpSnRK1β1的番茄株系T21、T23和超表达PpSnRK1β2的株系T91、T92。研究发现,与野生型(WT)相比,超表达株系T23和T91叶片的Sn RK1蛋白激酶活性分别增加9.84%和53.32%。T91叶片净光合速率比WT提高17.02%;叶片可溶性糖含量比WT增加34.00%。T91和T92叶片淀粉含量分别约是WT的2倍。T23、T91和T92果实维生素C含量分别比WT下降19.21%、38.49%和39.76%。因此说明PpSnRK1β1和PpSnRK1β2参与了光合作用过程,调控碳代谢及次生代谢过程。PpSnRK1 kinase 13 subunit encoding gene （PpSnRK1β1 and PpSnRKlβ2） were primarily cloned from peach EPrunus persica （Linn.） Batsch.] leaves. The CDS of PpSnRK1β and PpSnRKlfl2 are 720 bp and 867 bp respectively. Real-time quantitative polymerase chain reaction （qRT-PCR） analysis revealed that PpSnRKlβl expressed the highest in leaves of ＇Luxing＇ peach, while PpSnRKlβ2 expressed the highest in it＇s fruits, both expressed the lowest in it＇s flowers. By Agrobacterium mediated transformation, over-expression oftransgenic PpSnRKlβl （line T21 and T23 ） and PpSnRKlβ2 （line T91 and T92） tomato plants were obtained. Compared with wild type tomato （WT） activities of SnRK1 in young leaves of line T23 and T91 were increased by 9.84% and 53.32%, respectively. The photosynthetic rate in leaves of T91 was 17.02% higher than that of WT. The soluble sugar content in leaves was increased by ＇34.00% compared with WT. The starch content in leaves of T91 and T92 was nearly doubled compared with that in WT. Instead, the content of vitamin C in fruits of T23, T91 and T92 decreased by 19.21%, 38.49% and 39.76% compared with WT. These data demonstrate that PpSnRKlfll and PpSnRKlfl2 are involved in photosynthesis, and are regulated in carbohydrate metabolism and secondary metabolism.

关 键 词：桃 PpSnRKlfll PpSnRKlfl2 超表达 番茄 光合作用 碳代谢 

分 类 号：S662.1[农业科学—果树学]