半胱氨酸对氧化损伤小鼠胚胎抗氧化能力的影响  

Effects of cysteine on the antioxidant capacity of oxidative damaged mouse embryos

作　　者：徐礼杰[1] 柳海星[1] 李钟淑[1] 方南洙[1] 

机构地区：[1]延边大学农学院动物科学系,吉林延吉133000

出　　处：《黑龙江畜牧兽医》2017年第9期33-36,286,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31360546)

摘　　要：为了进一步了解活性氧(ROS)、半胱氨酸(Cys)与胚胎体外发育之间的关系,通过H2O2建立小鼠胚胎氧化损伤模型,采用2’,7’-二氯荧光素二乙酸盐(DCHFDA)法测定胚胎内部ROS水平,半定量PCR法测定小鼠胚胎内部NADPH氧化酶基因的表达。结果表明:胚胎经不同浓度半胱氨酸处理后,0.4 mmol/L半胱氨酸处理组的4-细胞率和囊胚率都显著比对照组高(P<0.05);氧化损伤胚胎经0.4 mmol/L半胱氨酸处理后,胚胎发育率显著高于H2O2处理组(P<0.05);DCHFDA法检测发现,H2O2+Cys处理组的ROS水平显著比H2O2处理组低(P<0.05);H2O2上调Nox3、Noxo1基因的表达(P<0.05),半胱氨酸下调Nox3、Noxo1基因的表达(P<0.05)。说明胚胎培养的最佳半胱氨酸添加浓度为0.4 mmol/L;半胱氨酸有效抑制NADPH氧化酶的活性,提高氧化损伤胚胎的抗氧化能力,利于胚胎发育。A study was conducted to research the relationship among ROS,cysteine and developmental capacity in early mouse embryos. The model of oxidative damage embryos was established by H_2O_2 treatment. The levels of ROS in embryos were measured by DCHFDA. The expression levels of NADPH oxidase were analyzed by semi-quantitative reverse transcription PCR. The results showed that levels of 0.4 mmol/L cysteine group was higher than control group in 4-cell rate and blastocyst rate( P<0.05). After H_2O_2 treatment,the levels of cysteine group was higher than H_2O_2group( P<0.05),and cysteine decreased the ROS level of embryos( P<0.05). H_2O_2 increased the levels of Nox3 and Noxo1expression( P<0.05),cysteine decreased the levels of Nox3 and Noxo1 expression( P<0.05). It was concluded that the optimumdose of cysteine for mouse embryo culturein vitro was 0.4 mmol/L. Cysteine decreased the activities of NADPH oxidase,and increased the antioxidant capacity of oxidative damage embryo,thus promoted embryo development.

关 键 词：半胱氨酸(Cys) 活性氧(ROS) NADPH氧化酶 氧化损伤 抗氧化作用 

分 类 号：S865.1[农业科学—野生动物驯养] S811.4[农业科学—畜牧兽医]