不同渗透压的稀释液对猪精液17℃保存效果的影响  

The effect of different osmotic pressures on the preservation of boar semen at 17 ℃

作　　者：李井春[1] 李琦[1] 唐德江[1] 纪新新 李雁冰[1] 黄大鹏[1] 李馨[1] 马群山[1] 魏国生[1] 

机构地区：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319

出　　处：《黑龙江畜牧兽医》2017年第9期118-120,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31402136);黑龙江省自然科学基金面上项目(JJ2017ZR0120);黑龙江省农垦总局科研计划项目(HNK135-04-06;HNK135-04-02-03);黑龙江省高等教育学会高等教育科学研究"十三五"规划项目(16G195;16G182);大庆市指导性科研项目(zd-2016-087);黑龙江八一农垦大学博士启动基金项目(B2012-06);黑龙江八一农垦大学教学改革课题项目(NDJY1610;NDJY1612)

摘　　要：为了研究猪精子在17℃保存时不同渗透压对精子质量、畸形率的影响,试验添加不同剂量的葡萄糖控制稀释液的渗透压,用迈朗全自动精子质量检测仪检测猪精子质量指标及畸形率。结果表明:在多数项目上,无论是保存时间还是质量参数(精子活率、精子活力、路径速度等),渗透压268,368 m Osmol/kg的2,3组都明显优于渗透压183,400 m Osmol/kg的1,4组。说明猪精子在等渗和适当高渗情况下有利于保存。To study the effect of different osmotic pressures on sperm quality and deformity rate at 17℃,in this experiment different proportions of glucose were added to controll diluents＇ osmotic pressure, and boar sperm quality and deformity rate were determined by using Mailang auto- matic sperm quality detector. The experimental data showed that, both the preservation time and the quality parameters （sperm motility rate, sperm motility, path velocity, etc）in group 2 and 3 （with osmotic pressure of 268 and 368 mOsmol/kg respectively）were obviously better than those in group 1 and 4 （with osmotic pressure of 183 and 400 m Osmol/kg each）. Those results demonstrated that iso-osmotic pressure or slightly high - osmotic pressure were beneficial to the preservation of pig sperm.

关 键 词：渗透压 稀释液 猪精液 17℃保存 精子质量 

分 类 号：S828[农业科学—畜牧学] S814[农业科学—畜牧兽医]