牦牛KLF8基因剪切体克隆及组织表达分析  

Cloning and tissue expression analysis of KLF8 Gene Spliced Variant in Yak(Bos grunniens)

作　　者：林森[1,2] 林亚秋 朱江江[2] 柏雪 江明锋[1] 王永[1,2] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]青藏高原动物遗传资源保护与利用四川省重点实验室,成都610041

出　　处：《黑龙江畜牧兽医》2017年第9期230-233,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：科技部科技支撑子项目(2014BAD13B03);四川省青年科技创新团队项目(2015TD0025)

摘　　要：为了阐明KLF8基因在牦牛不同组织中的表达水平,试验采用RT-PCR方法克隆KLF8基因剪切体序列,利用荧光定量PCR技术检测该基因在不同组织中的表达丰度。结果表明:获得牦牛KLF8基因剪切体序列长度为1 246 bp(Gen Bank登录号为KX964629),其中CDS为963 bp,编码320个氨基酸,与牛(登录号为XP_010820342.1)的氨基酸同源性达99.69%,无信号肽剪切位点和跨膜结构域,具有KLFs家族保守的锌指功能结构域;KLF8基因在牦牛的脂肪组织中存在高水平表达,极显著高于其他组织(P<0.01)。To elucidate the expression levels of KLF8 gene in different tissues of yak, the Reverse Transcription PCR （ RT - PCR） was used to clone yak KLF8 gene and the Real -time quantitative PCR（qPCR） was used to detect the expression levels of KLF8 gene in different tissues. The results showed that the length of KLF8 gene spliced variant was 1 246 bp（ GenBank accession No. KX964629） ,containing 963 bp in CDS region which encoded 320 amino acids. Amino acid homology of yak KLF8 with bovine（ accession No. XP_010820342.1 ） was 99.69% ,without signal peptide cleavage site and transmembrane domain but having a conserved zinc finger functional domains of KLFs family. The KLF8 gene had an high expression level in the adipose tissue of yak, significantly higher than in other tissues （P 〈 0.01 ）.

关 键 词：牦牛 KLF8基因 剪切体 克隆 组织表达 

分 类 号：S823.85[农业科学—畜牧学] Q785[农业科学—畜牧兽医]