苗药马桑狗帮、草狗肾不同部位的提取分离及体外抗风湿分子靶点初步筛选  

Extraction and isolation of different parts of the kidney of the dog, the dog and the dog, and the preliminary screening of the molecular targets of anti rheumatic in vitro

作　　者：刘益臻[1] 何康[1] 马武开[1] 杜江[1] 

机构地区：[1]贵阳中医学院药学院,贵州贵阳550022

出　　处：《影像研究与医学应用》2017年第4期20-23,共4页Journal of Imaging Research and Medical Applications

基　　金：贵州省科学技术基金(编号:黔科合J字[2015]2024)

摘　　要：目的:考察苗药马桑狗帮、草狗肾不同提取部位的体外抗风湿活性。方法:对马桑狗帮和草狗肾使用不同溶剂提取其化学物质,使用这些不同部位的不同浓度提取物体外干预在类风湿性关节炎的病理发展中起到重要作用的NF-κb,TNF-α,TNFR1,VEGFR1四种重组蛋白,利用酶联免疫分析方法检测药物对蛋白的体外抑制作用。结果:本研究共提取分离出8种不同部位的提取物,每种提取物对NF-κb,TNF-α,TNFR1,VEGFR1均有一定的体外抑制作用,马桑狗帮99%二氯甲烷洗脱部分对四种细胞因子的抑制作用均较强。结论:通过初步提取分离和初步筛选,两种苗药均有体外抗风湿的活性,其中马桑狗帮99%二氯甲烷洗脱部位可能存在某种化学物质具有较好的抗风湿活性。Objective To investigate the in vitro anti rheumatic activity of different extracts from the horse and the dog and the dog kidney.Method Coriaria dogs and grass dog kidney extract the use of chemical substances in different solvents, different concentrations of TNFR1 extract intervention in the pathogenesis of rheumatoid arthritis plays an important role in NF- kappa B, TNF- alpha, the use of these different parts, four kinds of recombinant protein VEGFR1 by enzyme immunoassay in vitro method for the detection of drugs on protein inhibition. Restflt The results of this study is to extract 8 different parts of the extracts of each extract on NF- kappa TNF- alpha, B, TNFR1, VEGFR1 have a certain inhibitory effect in vitro, Coriaria dogs 99% inhibition of dichloromethane extraction of four kinds of cytokines were strong.Conclusion The extraction and preliminary screening, two seedlings were activated in vitro anti rheumatism medicine, which help the 99% dogs of Coriaria dichloromethane may exist some elution chemicals has good anti rheumatic activity.

关 键 词：药物筛选 类风湿性关节炎 落新妇 苗药 

分 类 号：R285[医药卫生—中药学]