混合酶酶解法对天龙粉水提液抗肿瘤作用影响的研究  

Study on Anti-tumor Effect of Mixed Enzyme Enzymolysis for Water Extraction Liquid from Gecko Powder

作　　者：朱明珠[1] 王颖[1] 张由芹 王彦多[1] 王集会[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学实验中心,山东济南250355

出　　处：《山东化工》2017年第16期11-12,共2页Shandong Chemical Industry

基　　金：山东中医药大学大学生研究训练(SRT)计划资助项目(项目编号2016031)

摘　　要：目的:比较天龙粉水提液经混合酶酶解前后对喉癌hep2细胞株的抑制作用。方法:使用MTT法,以细胞抑制率为指标,研究天龙粉水提液经胰蛋白酶和弹性蛋白酶作用前后对喉癌hep2细胞株的抑制作用。结果:在所选药物浓度范围内,天龙粉水提液对喉癌hep2细胞株无抑制作用,一定浓度的天龙粉酶解液对喉癌hep2细胞株有抑制作用。结论:天龙粉水提液经胰蛋白酶和弹性蛋白酶酶解后在一定药物浓度下产生抑制喉癌hep2细胞株生长的作用。Objective ： To compare water extract from Gecko powder by mixed enzyme enzymolysis before and after have inhibition effect on laryngeal cancer cell hep2 line. Methods ： Using MTT method,the inhibition rate on cells as indicator,comparing the inhibitory effect on laryngeal cancer cell hep2 line for water extract from Gecko powder by trypsin and elastase function before and after. Results： Within the scope of the selected drug concentration,water extract from Gecko powder laryngeal haven＇t inhibition on laryngeal cancer cell hep2 line,enzymolysis water extract from Gecko powder in certain concentration have inhibition on laryngeal cancer cell hep2 line. Conclusion： Water extract from Gecko powder in certain concentration produce inhibition on laryngeal cancer cell hep2 line by trypsin and elastase function.

关 键 词：天龙粉 MTT法 喉癌hep2细胞株 混合酶 

分 类 号：R285.5[医药卫生—中药学]