高效液相色谱法同时测定兽用复方氨基比林注射液中二组分含量的研究  

Simultaneous Determination of Two Components in Veterinary Compound Aminophenazone Injection by HPLC

作　　者：陈锡龙[1] 孙真峥 王庆红[1] 

机构地区：[1]贵州省兽药饲料监察所,贵阳550003

出　　处：《中国兽药杂志》2017年第9期56-61,共6页Chinese Journal of Veterinary Drug

摘　　要：建立了兽用复方氨基比林注射液二组分含量测定的高效液相色谱法。以C18柱为固定相,0.05 mol/L的磷酸二氢钾(用三乙胺调节p H值到8.2)︰乙腈=75︰25为流动相,流速为1.0 m L/min,柱温30℃,检测波长240 nm。采用外标法定量计算含量。巴比妥的线性范围为5.6~280μg/m L,回归方程为y=21.856x+42.143(n=5),相关系数R2=1.0000;氨基比林的线性范围为14.3~715.0μg/m L,回归方程为y=22.267x-13.009(n=5),相关系数R2=1.0000。巴比妥80%、100%、120%三个添加水平的回收率在97.86%~101.06%之间,相应的氨基比林的回收率在99.48%~100.84%之间。方法专属性强,灵敏度佳,准确度高,能够有效控制兽用复方氨基比林注射液的质量。A method was developed to determine two components of veterinary Compound Aminophenazone Injection by HPLC, with C18 column as stationary phase, a solution of 0.05 mol/L potassium dihydrogen phosphate （pH adjusted to 8.2 by triethylamine）-acetonitrile = 75 ： 25 as mobile phase,flow rate of 1.0 mL/min, column temperature of 30 degree Celsius, and wavelength of 240 nm. It was quantified by external standard method and the linear range of barbital was between 5.6 and 280.0 μg/mL, and the regression equation was y= 21.856x＋42.143,R2 = 1.0000; the linear range of aminophenazone was between 14.3 and 715.0 μg/mL, and the regression equation was y= 22.267x-13.009, R2= 1.0000. Results showed that recoveries of 80%, 100%, 120% adding level of barbital were between 97.86% and 101.06%, and relative recoveries of aminophenazone were between 99.48% and 100.84%. The method was precise, specific and accurate, and could be used for the content determination of veterinary Compound Aminophenazone Injection.

关 键 词：复方氨基比林注射液 含量测定 高效液相色谱法 

分 类 号：S859.79[农业科学—临床兽医学]