血管紧张素(1-7)对CD40及CD40配体信号通路及活化黏附分子的抑制作用  

Inhibitory effect of Ang 1-7 on expression of CD40/CD40L pathway and activated adhesion molecules

作　　者：张利峰[1] 刘吉祥[2] 孙密欣 谷世奎[1] 石俊岭[3] 赵瑞芳[1] 

机构地区：[1]邯郸市第一医院心血管内二科,056000 [2]邯郸市第一医院脑外科,056000 [3]邯郸市第一医院中心实验室,056000

出　　处：《中华老年心脑血管病杂志》2017年第9期974-978,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

摘　　要：目的观察血管紧张素1-7[Ang-(1-7)]对血管紧张素Ⅱ(AngⅡ)诱导的人脐静脉内皮细胞中细胞间黏附分子1(ICAM-1)和血管细胞黏附分子1(VCAM-1)表达的影响及作用机制是否与CD40及CD40配体(CD40L)通路有关。方法人脐静脉内皮细胞培养后,分6组:对照组;AngⅡ组;低、中和高剂量组[Ang-(1-7)10、100和1000nmol/L预处理];阻断剂组(A-779预处理)。用RT-PCR法检测ICAM-1、VCAM-1和CD40、CD40L mRNA表达。结果与对照组比较,AngⅡ组ICAM-1、VCAM-1和CD40、CD40L mRNA表达显著升高(P<0.05);与AngⅡ组比较,低、中和高剂量组ICAM-1、VCAM-1和CD40、CD40L mRNA的表达逐渐下降(P<0.05),其中ICAM-1、VCAM-1mRNA表达分别下降25%、58%、69%和30%、53%、62%;CD40、CD40L mRNA表达分别下降35%、48%、61%和26%、54%、68%;阻断剂组与AngⅡ组上述指标比较无显著差异(P>0.05)。结论 Ang-(1-7)可以抑制炎症通路CD40/CD40L活化,进而下调黏附分子ICAM-1、VCAM-1的表达;Ang-(1-7)的抗炎机制是首先与Mas受体结合,进而发挥抑制CD40/CD40L通路的作用。Objective To study the inhibitory effect of Ang 1-7on expression of AngⅡ-induced intercellular adhesion molecule-1（ICAM-1）and vascular cell adhesion molecule-1（VCAM-1）in cultured human umbilical vein endothelial cells（HUVEC）and whether its mechanism is related with the CD40/CD40 Lpathway.Methods Cultured HUVEC were divided into control group,AngⅡgroup,low AngⅡdose（10nmol/L）group,medium AngⅡdose（100nmol/L）group,high AngⅡdose（1000nmol/L）group and blocker（A-779）group.Expressions of ICAM-1,VCAM-1,CD40/CD40 LmRNA were detected by RT-PCR.Results The expression levels of ICAM-1,VCAM-1,CD40/CD40 LmRNA were significantly higher in AngⅡgroup than in control group（P〈0.05）and were significantly lower in low AngⅡdose group,medium AngⅡdose group and high AngⅡdose group than in Ang Ⅱgroup（P〈0.05）.The expression of ICAM-1and VCAM-1decreased by 25%,58%,69% and 30%,53%,62%,and that of CD40/CD40 L mRNA decreased by 35%,48%,61% and 26%,54%,68%.No significant difference was found in the expressions of ICAM-1,VCAM-1,CD40/CD40 LmRNA between Ang Ⅱ group and blocker group（P〉0.05）.Conclusion Ang 1-7can inhibit the activation of CD40/CD40 Lpathway and downregulate the expression of ICAM-1and VCAM-1in HUVEC by binding to their specific receptor Mas.

关 键 词：血管紧张素类 血管紧张素Ⅱ 胞间黏附分子1 血管细胞黏附分子1 CD40配体 内皮细胞 

分 类 号：R541.4[医药卫生—心血管疾病]