黄芪多糖/壳聚糖缓释微球对大鼠变应性鼻炎的治疗作用  

Therapeutic effect of astragalus polysaccharides/chitosan microspheres on rats with allergic rhinitis

作　　者：孙玉卿[1] 李文涛[1] 刘康[2] 赵立民[3] 张维芬[2] 

机构地区：[1]潍坊医学院临床医学院,山东潍坊261053 [2]潍坊医学院药学院,山东潍坊261053 [3]潍坊医学院附属医院耳鼻咽喉科,山东潍坊261031

出　　处：《山东大学学报（医学版）》2017年第9期60-65,共6页Journal of Shandong University:Health Sciences

基　　金：国家科技部星火科技计划(2013GA740103);国家自然科学基金(81573717);山东省自然科学基金(ZR2012CM025);山东省科技攻关(2012YD18063);山东省医药卫生发展计划(2015WS0057)

摘　　要：目的观察黄芪多糖/壳聚糖缓释微球对大鼠变应性鼻炎的治疗作用。方法采用喷雾干燥法制备黄芪多糖/壳聚糖缓释微球,通过载药量、粒径、微球形态对微球进行表征。将60只健康Wistar大鼠随机分为正常组,模型组,布地奈德组,黄芪多糖/壳聚糖缓释微球低、中、高剂量组。用氢氧化铝凝胶腹腔注射和卵清蛋白滴鼻的方法制备变应性鼻炎大鼠模型,布地奈德组给予布地奈德溶液连续滴鼻,黄芪多糖/壳聚糖缓释微球低、中、高剂量组分别将微球混悬于生理盐水后给予连续滴鼻,模型组和正常组分别以生理盐水代替滴鼻。观察药物对大鼠变应性鼻炎症状评分及鼻黏膜病理组织改变的影响。结果黄芪多糖/壳聚糖缓释微球能有效抑制大鼠变应性鼻炎症状,减轻鼻炎的病理学改变。结论黄芪多糖/壳聚糖缓释微球对大鼠变应性鼻炎有明显的治疗作用。Objective To explore the therapeutic effect of astragalus polysaccharides/chitosan microspheres on rats with allergic rhinitis.Methods Astragalus polysaccharides/chitosan microspheres were prepared with spray drying method,and the physicochemical properties of microspheres were characterized by drug loading,particle size and morphology.A total of 60 healthy Wistar rats were randomly divided into the following groups：normal group,model group,budesonide group,astragalus polysaccharides/chitosan microspheres low-dose group,moderate-dose group and high-dose group.Allergic rhinitis rat models were built by intraperitoneal injection of aluminum hydroxide gel and intranasal administration of ovalbumin.The budesonide group was given budesonide solution by continuous nasal administratio;the polysaccharides/chitosan microspheres low-dose group,moderate-dose group and high-dose group were treated with saline solution of astragalus polysaccharide/chitosan microspheres with different dosages;the model group and normal group were treated with normal saline.The effects of the drugs on syndrome scores and pathological changes were observed.Results The symptoms of allergic rhinitis and pathological changes were effectively inhibited or reduced by astragalus polysaccharides/chitosan microspheres.Conclusion The astragalus polysaccharides/chitosan microspheres have significant therapeutic effect on allergic rhinitis in rats.

关 键 词：变应性鼻炎 黄芪多糖 壳聚糖 微球 鼻腔给药 

分 类 号：R944[医药卫生—药剂学]