元胡止痛方对小鼠疼痛模型的镇痛作用及其“量-效”关系探索研究  

Study on Analgesic Effect and Dose-Effect Relationship of Yuanhuzhitong Prescrption on Mice Model of Pain

作　　者：李俊芳[1] 王鹏 翟兴[3] 肖学凤[1] 张毅[4] 李德凤[4] 许海玉[4] 杨洪军[4] 

机构地区：[1]天津中医药大学中药学院,天津300193 [2]陶朱翁(天津)生物医药科技有限公司,天津300193 [3]北京中医药大学,北京100700 [4]中国中医科学院中药研究所,北京100700

出　　处：《现代中药研究与实践》2017年第3期21-24,共4页Research and Practice on Chinese Medicines

基　　金：国家科技支持计划课题(2011BAI07B00;2011BAI07B08);国家自然科学基金面上项目(81202793)

摘　　要：目的研究元胡止痛方对小鼠疼痛模型的镇痛作用及其"量-效"关系探索。方法将健康ICR小鼠随机分为5个组,采用热板模型记录反应潜伏期和醋酸扭体模型观察扭体潜伏期及注射醋酸后20min内的扭体次数。用归一化法探索其"量效"关系。结果在热板试验中,与模型组比较,给药60 min后,阳性药组与元胡止痛方16 g/kg和32 g/kg生药组痛阈时间明显延长(P<0.05)。在醋酸扭体试验中,与模型组比较,阳性药及各元胡止痛方组扭体次数明显减少(P<0.05)。归一化结果说明,在4~32 g/kg剂量范围内,元胡止痛方的剂量与其提高的痛阈值成正相关。结论元胡止痛方对小鼠疼痛模型有明显的镇痛作用,且呈现出剂量依赖性关系。Objective To explore the analgesic effect and dose-effect relationship of Yuanhuzhitong prescrption on mice model of pain. Methods ICR mice were randomly divided into 5 groups. On hot-plate test in mice, the changes of the latencies of licking paws were observed. Moreover, the latent period and the number of writhing in 20 min were calculated in writhing test. Adoption normalization method to expore the dose-effect relationship. Results Compared with the model group, positive drug group and Yuanhuzhitong prescripton group were signifi cantly increased（P 〈 0.05） after adminstration 60 min. In writhing test, Yuanhuzhitong prescripton can prolonged latent period of twisting and decreased the number of times of twisting（P 〈 0.05）. There were highpositive correlation between dose of Yuanhuzhitong prescripton and effect of increasing threshold of pain within 4 ~ 32 g/kg dose range. Conclusion Yuanhuzhitong prescrption had signifi cantly analgesic effect on pain pattern and with dose related.

关 键 词：元胡止痛方 镇痛作用 醋酸扭体模型 热板模型 量效关系 

分 类 号：R285.5[医药卫生—中药学]