洋金花单体化合物对CD4+T细胞亚群的影响  

Effect of Yangjinhua Compounds on CD4^+T Cell Subsets

作　　者：苏阳[1,2] 张雪婷[1] 杨炳友[1] 程根宏[2] 王秋红[1] 陈晶[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学药学院中药药效物质基础省部共建重点实验室,黑龙江哈尔滨150040 [2]美国加州大学洛杉矶分校微生物与免疫遗传学院,losangelesca90095

出　　处：《现代中药研究与实践》2017年第3期25-28,共4页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金(81603416);中国博士后科学基金资助项目(2016M600267);第七批黑龙江省博士后特别资助(博士后青年英才计划:LBH-TZ1619)~~

摘　　要：目的证明洋金花单体化合物对T细胞亚群细胞因子的影响。方法采用MTT比色法测定各化合物对小鼠脾淋巴细胞的毒性,以及混合淋巴细胞增殖反应,及对CD4^+T细胞亚群的影响,采用PCR扩增技术对各细胞因子mRNA进行扩增,对结果进行统计学处理。结果化合物G4有较高的细胞毒性浓度值,并且可强烈抑制T细胞增殖,对Th17细胞和Treg细胞有调节作用。结论洋金花G4对T细胞的增殖有抑制作用,主要可抑制CD4^+T细胞中Th17和Treg细胞相关转录因子的表达。Objective To prove the effect of Yangjinhua compounds on cytokine T cell subsets. Methods The determination of toxic compounds on mouse spleen lymphocyte MTT assay, and mixed lymphocyte reaction, compound G4 on spleen lymphocyte proliferation inhibition. PCR amplification factor was used to amplify m RNA, and the results were statistically analyzed. Results Compound G4 cytotoxicity concentration value, and could strongly inhibit the proliferation of T cells, the regulatory role of Th17 cells and Treg cells. ConclusionThe inhibitory effect of Datura G4 on proliferation of T cells, which can inhibit CD4~＋ T cells in Th17 and Treg cell related transcription factor Expression.

关 键 词：洋金花醉茄内酯类化合物 银屑病 CD4^+T淋巴细胞 Th17/Treg比例 

分 类 号：R285.5[医药卫生—中药学]