HPLC-DAD同时测定洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-O-β-D葡萄糖苷的含量  

Simultaneous Determination the Contents of Galuteolin-7-O-β-D-Glucoside and Apigenin-7-O-β-D Glucoside in Matricaria recutita by HPLC-DAD

作　　者：游龙泰 董晓旭[1] 冷新[1] 王美玲[1] 尹兴斌[1] 倪健[1] 

机构地区：[1]北京中医药大学中药学院,北京100029

出　　处：《现代中药研究与实践》2017年第4期23-25,共3页Research and Practice on Chinese Medicines

基　　金：北京中医药大学协同创新建设计划(2013-XTCX-03)

摘　　要：目的建立同时测定洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素7-O-β-D葡萄糖苷含量测定的HPLC方法。方法色谱柱:Agilent Zorbax SB-C_(18)(4.6 mm×250 mm,5μm);流动相:甲醇-0.2%磷酸(34∶66);流速:1.0 ml/min;柱温25℃;进样量10μl;检测波长:350 nm。结果木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-0-β-D葡萄糖苷的线性范围分别为5.11~409.00、12.56~1 005.00μg/ml,相关系数r>0.999 3,具有良好的线性。精密度、稳定性符合要求,木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-0-β-D葡萄糖苷加样回收率分别为96.44%~103.55%、95.12%~101.52%,RSD均为0.03%。结论该方法简便、准确、重复性好,适用于洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素7-O-β-D葡萄糖苷含量测定。Objective To establish an HPLC method for simultaneous determination the contents of galuteolin and apigenin 7-O-β-D glucoside in Matricaria recutita simultaneously. Methods The contents of galuteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside were determined on an Agilent Zorbax SB-C18 column （4.6 mm x 250 mm, 5 pm） with a mobile phase consisting of methanol-0.2% phosphoric acid （34 ： 66） at a flow rate of 1.0 ml/min and detected at a wavelength of 350 nm, column temperature was 30℃. Results The linear range of galuteolin -7-0-β0 glucoside and apigenin-7-0-β-D glucoside was 5.11 - 409.00, 12.56 - 1 005.00μg/ml, the correlation coefficient r 〉 0.999 3, has a good linearity. Precision and stability meet the requirements, the recovery was 96.44% - 103.55 % and 95.12%-101.52%, for galuteolin-7-O-β-D-glucoside, apigenin-7-O- 13-D-glucoside, respectively. Conclusion The method is simple, selective and has good repeatability. It can be used for the simultaneous determination of galuteolin-7-O-β-D-glucoside and apigenin-7-O-β-D -glucoside in Matricaria recutita.

关 键 词：木犀草素-7-O-β-D葡萄糖苷 芹菜素-7-O-β-D葡萄糖苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]