碧玉散质量标准提高研究  

Study on Improving the Quality Standard of Biyu Powder

作　　者：管敏[1] 达庆国[1] 

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《现代中药研究与实践》2017年第4期63-66,共4页Research and Practice on Chinese Medicines

基　　金：江苏省中药饮片炮制规范修订专项

摘　　要：目的提高碧玉散的质量标准。方法采用薄层色谱法定性鉴别处方中甘草、青黛药材;用高效液相色谱法测定靛玉红的含量,流动相为甲醇∶水(70∶30);流速:1.0 ml/min;柱温:30℃;检测波长:292 nm。结果甘草、青黛薄层鉴别图清晰,分离度好,阴性对照无干扰;靛玉红回归方程为:Y=2 837.4 X+0.664 93(r=0.999 9),靛玉红在0.025 2~0.25 2μg范围内其峰面积值呈良好的线性关系;平均回收率为99.23%,RSD=1.54%(n=6)。结论薄层鉴别结果清晰、专属性强;含量测定方法简便、重现性好,为碧玉散质量控制提供了依据。Objective To improve the quality standard of Biyu powder. Methods Identify Licorice, Indigo herbs of the prescription by thin layer chromatography. Determine the content of the indirubinby by high-performance liquid chromatography. Mobile phase is methanol and water （70 ： 30）;Velocity of flow is 1.0 ml/min;The column temperature is 30 ~C; The wavelength of detector is 292 nm. Results The regression equation of the indirubin is Y = 2 837.4 X ＋ 0.664 93 （ r = 0.999 9）. Its peak area values showed a good linear relationship within the scope of 0.025 2 - 0.252 0μg. The average recovery rate is 99.23%, RSD = 1.54 % （n = 6）. Conclusion TLC results are distinct and specific. Content determination method is simple and easy to reproduce. It can provide the basis for controlling the quality of Biyu powder.

关 键 词：碧玉散 青黛 靛蓝 靛玉红 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]