硒化麒麟菜多糖对膀胱癌细胞生长、凋亡的作用及Bax、Bcl-2、caspase-3蛋白表达的影响  

Effects of seleno-polysaccharide on the growth and apoptosis of bladder cancer cells and protein expressions of Bax,Bcl-2 and caspase-3

作　　者：刘安[1] 刘龙强[1] 张建勋[1] 

机构地区：[1]延安市人民医院泌尿外科,陕西延安716000

出　　处：《现代泌尿外科杂志》2017年第9期700-704,共5页Journal of Modern Urology

摘　　要：目的研究硒化麒麟菜多糖对膀胱癌T24细胞生长、凋亡的作用及Bax、Bcl-2、caspase-3蛋白表达的影响。方法体外培养T24膀胱癌细胞,分别利用0、0.125、0.25、0.5、1 mg/mL的硒化麒麟菜多糖处理T24细胞24h、48h、72h,利用噻唑蓝(MTT)检测各组细胞增殖情况,0.25、0.5、1mg/mL的硒化麒麟菜多糖处理细胞48h后,流式细胞仪检测T24细胞周期分布及细胞凋亡,Western-blot检测T24细胞Bcl-2相关X蛋白(Bax)、B细胞淋巴瘤/白血病-2(Bcl-2)、活化的含半胱氨酸的天冬氨酸蛋白水解酶3(Cleaved caspase-3)蛋白表达。结果与0mg/mL处理相比,0.25、0.5、1mg/mL的硒化麒麟菜多糖处理抑制T24细胞增殖,差异有非常显著性统计学意义(P<0.01);使用0.125mg/mL硒化麒麟菜多糖处理,作用24h时对细胞增殖抑制不显著(P>0.05),作用48h、72h时对细胞增殖具有较为明显抑制作用(P<0.05)。1mg/mL的硒化麒麟菜多糖作用后细胞凋亡率高达(23.56±0.96)%。与0 mg/mL处理相比,0.25、0.5、1 mg/mL的硒化麒麟菜多糖作用后,处于G0/G1期的细胞从(52.12±1.74)%分别增加至(64.32±2.42)%、(70.72±1.71)%、(76.09±2.28)%。处于G2/M期和S细胞明显减少(P<0.05),Bcl-2蛋白的表达明显降低(P<0.01),Bax、Cleaved-caspase-3蛋白的表达明显升高(P<0.01)。结论硒化麒麟菜多糖可能通过调控Bax、Bcl-2、Cleaved-caspase-3蛋白表达抑制T24细胞增殖,阻滞细胞周期进程,促进细胞凋亡。Objective To investigate the effects of seleno-polysaccharide on the growth and apoptosis of bladder cancer cells and on the protein expressions of Bcl-2 ,Bax arid caspase-3. Methods Bladder cancer cell line T24 were cultured in vitro, treated with 0 mg/mL,0. 125 mg/mL,0.25 mg/mL,0.5 mg/mL,and 1 mg/mL seleno-polysaccharide for 24 h,48 h and 72 h re- spectively. The cell proliferation was detected with MTT. After the cells were treated for 48 h,cell cycle and apoptosis were de- tected with flow cytometry. The protein expressions of Bax, Bcl-2 and caspase-3 were determined with Western blotting. Results Seleno-polysaccharide at concentrations of 0.25 mg/mL,0.5 mg/mL,and 1 mg/mL significantly inhibited cell prolifera- tion （P〈0.01）. Seleno-polysaccharide at 0. 125 mg/mL for 24 h had no significant inhibitive effect on cell proliferation （P〉 0.05） ,while the 48 h and 72 h treatment had a significant inhibitive effect （P^0.05）. The cell apoptosis rate was （23.56±0.96） % after treatment of seleno-polysaccharide at 1 mg/mL. After seleno-polysaccharide treatment at concentrations of 0.25, 0.5 and 1 mg/mL,the percentage of cells in G0/G1 phase rose from （52.12± 1.74）% to （64.32±2.42） %,（70.72±1.71）% and （76.09±2.28）%; percentage of cells in G2/M and S phase decreased significantly （P〈0.05）. The protein expression of Bcl-2 significantly reduced,while the protein expressions of Bax and caspase3 significantly increased （P〈0.01）. Conclusion Seleno-polysaccharide inhibits T24 cell proliferation,arrests cell cycle,and promotes cell apoptosis by regulating the expressions of Bax,Bcl-2 and caspase-3.

关 键 词：硒化麒麟菜多糖 膀胱癌细胞 增殖 细胞周期 凋亡 

分 类 号：R694[医药卫生—泌尿科学]