气-质谱联用法测定罗汉果中有机磷农药残留  

Determination of Organophosphorous Pesticide Residues in Siraitiae Fructus by GC-MS

作　　者：白红妍[1] 付明磊[1] 罗琴[1] 周先丽[1] 黄晓[1] 苏小建[2] 程柳[3] 李远远[1] 梁成钦[1] 

机构地区：[1]桂林医学院广西高校生药学重点实验室,广西桂林541004 [2]广西师范大学环境与资源学院,广西桂林541004 [3]中国有色桂林矿产地质研究院有限公司,广西桂林541004

出　　处：《食品研究与开发》2017年第17期139-142,共4页Food Research and Development

基　　金：广西科学研究与技术开发计划项目(桂科能14123006-21;桂科能1598025-18)

摘　　要：建立气相色谱-质谱联用法(gas chromatography-mass spectrometry,GC-MS)测定罗汉果中11种常用有机磷农药残留量的方法。样品经石油醚-丙酮(体积比2∶1)超声提取,提取物通过活性炭净化后选用HP-5MS毛细管柱对11种有机磷农药进行分离,GC-MS检测,外标法计算含量。11种有机磷农药在线性范围内(10μg/L^750μg/L),相关系数均大于0.999 0,检出限为LOD为0.432μg/L^3.901μg/L。样品3个加标水平(0.125、0.25、0.375μg/g)下,罗汉果中11种有机磷农药回收率均在83.3%~112.4%,相对标准偏差(RSD,n=6)为1.45%~8.28%。A method for the determination of 11 organophosphorus pesticides in Siraitiae Fructus using gas chromatography-mass spectrometry（GC-MS）was developed.Ultrasonic was used to extract the organophosphorous pesticides by petroleum and acetone（volume ratio=2：1）. After the clean-up with active carbon,the extract was ready for GC-MS analysis with the external standard method. The linear equations were obtained in the concentration range of 10 μg/L-750 μg/L with correlation coefficients greater than 0.999 0. The limits of detection（S/N=3）were 0.432 μg/L-3.901 μg/L. The average recovery at the three spiked levels of 0.125,0.25,0.375 μg/g range from 83.3 % to 112.4 % with the relative standard deviation（RSD,n=6）from 1.45 % to 8.28 %.

关 键 词：气质联用法 有机磷农药 罗汉果 

分 类 号：O657.63[理学—分析化学] TS207.53[理学—化学]