番茄SlGAMYBL2基因启动子的克隆及表达载体的构建  

Cloning of Sl GAMYL2 Promoter in Tomato and Construction of Plant Expression Vector

作　　者：李姗姗[1,2] 赖馥茜 蓝春莲 王小敏 莫昭展 

机构地区：[1]广西大学林学院,广西南宁530004 [2]广西高校桂东南珍稀经济物种保护利用重点实验室培育基地,广西玉林537000 [3]玉林师范学院生物与制药学院,广西玉林537000

出　　处：《玉林师范学院学报》2017年第2期70-74,共5页Journal of Yulin Normal University

基　　金：国家自然科学基金(31360485);2016年地方高校国家级大学生创新创业训练项目(201610606026)

摘　　要：采用5'端系列缺失分析法,利用PCR方法对Sl GAMYBL2基因上游序列进行特异性扩增,分别克隆1368bp、1127bp、990bp和730bp的启动子片段并构建到p LPlp100载体,经鉴定,构建了4个含Sl GAMYBL2启动子不同区段融合GUS报告基因的植物表达载体,通过农杆菌转化烟草愈伤组织,转化植株经PCR和GUS染色法鉴定,表明构建的表达载体已经转化进烟草植株,为确定Sl GAMYBL2基因上游片段中的顺式作用元件响应非生物胁迫诱导中的作用机理以及后期研究该启动子的组织特异性及诱导表达模式奠定基础.Using 5＇ end serial deletion analysis method, the specific promoter S1GAMYBL2 5＇ deletion promoter fragments were amplified by PCR. Promoter with a variation of length, 1386bp, 1145bp, 1008bp and 748bp, were ligated with pLP100 vector. After identification, expression vectors carrying these 4 S1GAMYBL2 promoters with deletion fragments driying β-glucuronidase gene （GUS） were constructed. Then the expression vector transformed into tobacco via Agrobacterium mediation. Transgenic tobacco plants were obtained by PCt（ amplification and GUS staining. These results may make an important basis for the investigation on expression character and the key cis-acting element of the promoter.

关 键 词：番茄 启动子 载体构建 顺式作用元件 

分 类 号：Q945.78[生物学—植物学]